Dataset Information


Promoter P PSP1-5-BnPSP-1 From Ramie (Boehmeria nivea L. Gaud.) Can Drive Phloem-Specific GUS Expression in Arabidopsis thaliana.

ABSTRACT: Isolation of phloem-specific promoters is one of the basic conditions for improving the fiber development and resistance of ramie phloem using genetic engineering. In this study, we isolated a ramie endogenous promoter (named P PSP1 -BnPSP-1) and analyzed the function of its truncated fragments in Arabidopsis. The results show that P PSP1 -BnPSP-1 can drive the GUS reporter gene to be specifically expressed in the veins of Arabidopsis. After hormone and simulated drought treatment of the independent Arabidopsis lines carrying P PSP1 -BnPSP-1 and its truncated fragments, only P PSP1-5-BnPSP-1 (-600 to -1 bp region of P PSP1 -BnPSP-1) is stably expressed and exhibits phloem specificity. Our findings suggest that P PSP1-5-BnPSP-1 can be used as a phloem specific promoter for further research.


PROVIDER: S-EPMC7772962 | BioStudies | 2020-01-01

REPOSITORIES: biostudies

Similar Datasets

2020-01-01 | S-EPMC6958601 | BioStudies
2014-01-01 | S-EPMC4213010 | BioStudies
2014-01-01 | S-EPMC4240604 | BioStudies
2013-01-01 | S-EPMC3846573 | BioStudies
2019-01-01 | S-EPMC6562848 | BioStudies
2016-01-01 | S-EPMC4789370 | BioStudies
2018-01-01 | S-EPMC6048116 | BioStudies
2018-01-01 | S-EPMC5947917 | BioStudies
2015-01-01 | S-EPMC4346909 | BioStudies
2020-01-01 | S-EPMC7039886 | BioStudies