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Synthesizing Ag⁺: MgS, Ag⁺: Nb₂S₅, Sm³⁺: Y₂S₃, Sm³⁺:Er₂S₃, and Sm³⁺:ZrS₂ Compound Nanoparticles for Multicolor Fluorescence Imaging of Biotissues.

ABSTRACT: Development of the fluorophores whose fluorescence bands can be flexibly selected is of great interest for biotissue imaging. Compounds of Ag⁺:MgS, Ag⁺:Nb₂S₅, Sm³⁺:Y₂S₃, Sm³⁺:Er₂S₃, and Sm³⁺:ZrS₂ were obtained through new chemical synthesis. They were characterized by X-ray photoelectron spectroscopy, X-ray diffraction spectroscopy, and transmission electron microscopy. They revealed polychromatic-photoluminescence spectra when excited by 280, 380, 480, 580, 680, and 785 nm light. Especially, near-infrared emission ranging from 800-1100 nm was found upon 785 nm light excitation. A band model was proposed to explain transitions responsible for the observed components of emission. Their broad fluorescence spectra cover from the ultraviolet to near-infrared spectral range. Their ability of emitting wide-range fluorescence was utilized for multicolor fluorescence imaging of biotissues, as demonstrated by pig-kidney tissue samples.

PROVIDER: S-EPMC7774074 | BioStudies |

REPOSITORIES: biostudies

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Project description:Reliable, label-free, and ultraselective detection of Pb2+ and Ag+ ions is of paramount importance for toxicology assessment, human health, and environmental protection. Herein, we present a novel recyclable fluorometric aptasensor based on the Pb2+ and Ag+-induced structural change of the GC-rich ssDNA (guanine cytosine-rich single-strand DNA) and the differences in the fluorescence emission of acridine orange (AO) from random coil to highly stable G-quadruplex for the detection of Pb2+ and Ag+ ions. More interestingly, the construction and principle of the aptasensor explore that the GC-rich ssDNA and AO can be strongly adsorbed on the CaSnO3@PDANS surface through the π-π stacking, hydrogen-bonding, and metal coordination interactions, which exhibit high fluorescence quenching and robust holding of the GC-rich ssDNA. However, in the presence of Pb2+, the specific G-rich ssDNA segment could form a stable G-quadruplex via G4-Pb2+ coordination and capture of AO from the CaSnO3@PDANS surface resulting in fluorescence recovery (70% enhancement). The subsequent addition of Ag+ ion induces coupled cytosine base pairs in another segment of ssDNA to get folded into a duplex structure together with the G-quadruplex, which highly stabilizes the G-quadruplex resulting in the maximum recovery of AO emission (99% enhancement). When the Cys@Fe3O4Nps are added to the above solution, the sensing probe was restored by complexation between the Cys in the Cys@Fe3O4Nps and target metal ions, resulting in the fabrication of a highly sensitive recyclable Pb2+ and Ag+ assay with detection limits of 0.4 and 0.1 nM, respectively. Remarkably, the Cys@Fe3O4Nps can also be reused after washing with EDTA. The utility of the proposed approach has great potential for detecting the Pb2+ and Ag+ ions in environmental samples with interfering contaminants.

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Synthesizing Ag+: MgS, Ag+: Nb2S5, Sm3+: Y2S3, Sm3+:Er2S3, and Sm3+:ZrS2 Compound Nanoparticles for Multicolor Fluorescence Imaging of Biotissues.

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Synthesizing Ag⁺: MgS, Ag⁺: Nb₂S₅, Sm³⁺: Y₂S₃, Sm³⁺:Er₂S₃, and Sm³⁺:ZrS₂ Compound Nanoparticles for Multicolor Fluorescence Imaging of Biotissues.