Unknown

Dataset Information

0

The MERS-CoV N Protein Regulates Host Cytokinesis and Protein Translation via Interaction With EF1A.


ABSTRACT: Middle East respiratory syndrome coronavirus (MERS-CoV), a pathogen causing severe respiratory disease in humans that emerged in June 2012, is a novel beta coronavirus similar to severe acute respiratory syndrome coronavirus (SARS-CoV). In this study, immunoprecipitation and proximity ligation assays revealed that the nucleocapsid (N) protein of MERS-CoV interacted with human translation elongation factor 1A (EF1A), an essential component of the translation system with important roles in protein translation, cytokinesis, and filamentous actin (F-actin) bundling. The C-terminal motif (residues 359-363) of the N protein was the crucial domain involved in this interaction. The interaction between the MERS-CoV N protein and EF1A resulted in cytokinesis inhibition due to the formation of inactive F-actin bundles, as observed in an in vitro actin polymerization assay and in MERS-CoV-infected cells. Furthermore, the translation of a CoV-like reporter mRNA carrying the MERS-CoV 5'UTR was significantly potentiated by the N protein, indicating that a similar process may contribute to EF1A-associated viral protein translation. This study highlights the crucial role of EF1A in MERS-CoV infection and provides new insights into the pathogenesis of coronavirus infections.

PROVIDER: S-EPMC8261062 | BioStudies |

REPOSITORIES: biostudies

Similar Datasets

| S-EPMC2446950 | BioStudies
| S-EPMC6158436 | BioStudies
2014-08-31 | E-GEOD-56677 | ArrayExpress
| S-EPMC7117464 | BioStudies
2021-11-16 | GSE188514 | GEO
2019-11-30 | GSE139516 | GEO
| S-EPMC5081173 | BioStudies
| S-EPMC4769073 | BioStudies
| S-EPMC8603276 | BioStudies
| S-EPMC3807420 | BioStudies