Unknown

Dataset Information

0

MicroRNA Expression Profiling in Porcine Liver, Jejunum and Serum upon Dietary DON Exposure Reveals Candidate Toxicity Biomarkers.


ABSTRACT: Deoxynivalenol (DON), a frequent mycotoxin worldwide, impairs human and animal health. The response of microRNAs, small non-coding RNAs, to DON has been scarcely investigated, but holds remarkable potential for biomarker applications. Hence, we aimed to investigate DON-induced changes in the microRNA expression in porcine liver, jejunum and serum by combining targeted and untargeted analyses. Piglets received uncontaminated feed or feed containing 900 µg/kg and 2500 µg/kg DON for four weeks, followed by a wash-out period. In tissue, only slight changes in microRNA expression were detected, with ssc-miR-10b being downregulated in liver of DON-exposed piglets. In serum, several microRNAs were differentially expressed upon DON exposure, four of which were validated by qPCR (ssc-miR-16, ssc-miR-128, ssc-miR-451, ssc-miR-205). The serum microRNA response to DON increased over time and declined after removal of contaminated diets. Receiver operating curve analyses for individual microRNAs were significant, and a combination of the four microRNAs increased the predictive capacity for DON exposure. Predicted microRNA target genes showed enrichment of several pathways including PIK3-AKT, Wnt/β-catenin, and adherens junctions. This study gives, for the first time, a comprehensive view of the porcine microRNA response to DON, providing a basis for future research on microRNAs as biomarkers for mycotoxins.

PROVIDER: S-EPMC8585098 | BioStudies |

REPOSITORIES: biostudies

Similar Datasets

| S-EPMC6598998 | BioStudies
2019-07-16 | GSE126989 | GEO
| S-EPMC6560770 | BioStudies
| S-EPMC5793127 | BioStudies
| S-EPMC6891430 | BioStudies
| S-EPMC4008308 | BioStudies
| S-EPMC6315731 | BioStudies
| S-EPMC3528764 | BioStudies
| S-EPMC4934789 | BioStudies
| S-EPMC6215154 | BioStudies