Unknown

Dataset Information

0

Tungsten Enzyme Using Hydrogen as an Electron Donor to Reduce Carboxylic Acids and NAD.


ABSTRACT: Tungsten-dependent aldehyde oxidoreductases (AORs) catalyze the oxidation of aldehydes to acids and are the only known enzymes reducing non-activated acids using electron donors with low redox potentials. We report here that AOR from Aromatoleum aromaticum (AOR Aa ) catalyzes the reduction of organic acids not only with low-potential Eu(II) or Ti(III) complexes but also with H2 as an electron donor. Additionally, AOR Aa catalyzes the H2-dependent reduction of NAD+ or benzyl viologen. The rate of H2-dependent NAD+ reduction equals to 10% of that of aldehyde oxidation, representing the highest H2 turnover rate observed among the Mo/W enzymes. As AOR Aa simultaneously catalyzes the reduction of acids and NAD+, we designed a cascade reaction utilizing a NAD(P)H-dependent alcohol dehydrogenase to reduce organic acids to the corresponding alcohols with H2 as the only reductant. The newly discovered W-hydrogenase side activity of AOR Aa may find applications in either NADH recycling or conversion of carboxylic acids to more useful biochemicals.

PROVIDER: S-EPMC9295118 | BioStudies |

REPOSITORIES: biostudies

Similar Datasets

| S-EPMC6365974 | BioStudies
| S-EPMC5620155 | BioStudies
| S-EPMC7484307 | BioStudies
| S-EPMC7161547 | BioStudies
| S-EPMC6367545 | BioStudies
| S-EPMC7175950 | BioStudies
| S-EPMC8578170 | BioStudies
| S-EPMC7018833 | BioStudies
| S-EPMC9306933 | BioStudies
| S-EPMC5765548 | BioStudies