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Outer membrane proteins Omp10, Omp16, and Omp19 of Brucella spp. are lipoproteins.


ABSTRACT: The deduced sequences of the Omp10, Omp16, and Omp19 outer membrane proteins of Brucella spp. contain a potential bacterial lipoprotein processing sequence. After extraction with Triton X-114, these three proteins partitioned into the detergent phase. Processing of the three proteins is inhibited by globomycin, a specific inhibitor of lipoprotein signal peptidase. The three proteins were radioimmunoprecipitated from [(3)H]palmitic acid-labeled Brucella abortus lysates with monoclonal antibodies. These results demonstrate that Omp10, Omp16, and Omp19 are lipoproteins.

SUBMITTER: Tibor A 

PROVIDER: S-EPMC96837 | BioStudies | 1999-01-01

REPOSITORIES: biostudies

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