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Carnoy’s Solution Versus Formalin in Rectal and Gastric Cancer Specimens Following: a Randomized Trial

ABSTRACT: This study evaluates the number of lymph nodes dissected in specimens following fixation with 10% neutral buffered formaldehyde or Carnoy’s solution. Specimens were randomized for fixation in each solution.

DISEASE(S): Adenocarcinoma,Gastric Adenocarcinoma,Rectal Cancer

PROVIDER: 2204199 | ecrin-mdr-crc |

REPOSITORIES: ECRIN MDR

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Project description:Interventions: Colorectal adenocarcinoma patients, consecutively submitted to surgical treatment performed by colorectal surgeons with more than 10 years of professional experience at Hospital Moinhos de Vento (Porto Alegre, Brazil), will have their surgical specimens subjected to conventional anatomopathological examination for the analysis of the lymph nodes (visual and palpation) performed by a highly qualified pathology technician in lymph node clearing technique. The lymph nodes identified in this preliminary examination will be counted and submitted to pathologic examination with hematoxylin and eosin staining for lymph node metastasis by a pathologist with over 10 years of experience. Upon completing this procedure with the patients with colon cancer, those with rectal cancer will be randomized and have their surgical specimens submitted to the lymph node clearing technique with Carnoy’s or GEWF solutions, according to the groups below. 1) Colon Cancer Group – Carnoy Number of participants: 38 individuals Procedure: The surgical resection specimen will be embedded in a plastic container with 1000 ml of Carnoy solution for 24 to 48 hours. Carnoy’s solution is a fixative composed of 60% ethanol, 30% chloroform and 10% glacial acetic acid. Following, a technician in pathological macroscopy with 10 years of experience will perform a visual and manual assessment of additional lymph nodes for 60 minutes. Each additional lymph node identified will be entirely or partially embedded, depending on the size, in paraffin and subsequently stained with hematoxylin-eosin.The microscopic examination of the slides obtained from the anatomopathological examination with Carnoy’s solution will be performed by two pathologists with seven and 25 years of experience, respectively;Other;H02.403.650.510 Primary outcome(s): Increased lymph node sample: additional number (unit measure) of lymph nodes identified by the lymph node clearing technique (visual and manual examination with Carnoy’s or GEWF solutions) compared to the initial number (unit measure) of lymph nodes identified by the conventional technique (visual and manual examination with formaldehyde). Study Design: Randomized clinical trial, controlled, single-blind, parallel with four groups.

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Carnoy’s Solution Versus Formalin in Rectal and Gastric Cancer Specimens Following: a Randomized Trial

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