Dataset Information

Microwave Ablation or Wedge Resection for the Treatment of Lung, Sarcoma and Colorectal Lesions, ALLUME Study

ABSTRACT: This study compares the outcomes and safety of two standard treatment options called microwave ablation and surgical wedge resection in patients with non-small cell lung cancer, sarcoma and colorectal cancer that has spread to other parts of the body (metastatic). Microwave ablation is designed to kill tumor cells by heating the tumor until the tumor cells die. A wedge resection is a procedure that involves the surgical removal of a small, wedge-shaped piece of lung tissue to remove a small tumor or to diagnose lung cancer. Comparing these two treatment options may help researchers learn which method works better for the treatment of non-small cell lung cancer, metastatic sarcoma, and metastatic colorectal cancer.

DISEASE(S): Stage Iva Colorectal Cancer Ajcc V8,Stage Iiic Lung Cancer Ajcc V8,Carcinoma,Stage Iib Lung Cancer Ajcc V8,Metastatic Colorectal Carcinoma,Lung Neoplasms,Stage Ii Lung Cancer Ajcc V8,Stage Iiia Lung Cancer Ajcc V8,Stage Ivb Colorectal Cancer Ajcc V8,Colorectal Neoplasms,Metastatic Sarcoma,Stage Ia2 Lung Cancer Ajcc V8,Carcinoma, Non-small-cell Lung,Stage Ivc Colorectal Cancer Ajcc V8,Stage Iii Lung Cancer Ajcc V8,Stage Iv Colorectal Cancer Ajcc V8,Sarcoma,Stage Iiib Lung Cancer Ajcc V8,Lung Non-small Cell Carcinoma,Stage Iva Lung Cancer Ajcc V8,Stage I Lung Cancer Ajcc V8,Stage Iia Lung Cancer Ajcc V8,Stage Ivb Lung Cancer Ajcc V8,Stage Ia1 Lung Cancer Ajcc V8,Stage Ib Lung Cancer Ajcc V8,Stage Iv Lung Cancer Ajcc V8,Stage Ia3 Lung Cancer Ajcc V8

PROVIDER: 2342482 | ecrin-mdr-crc |

REPOSITORIES: ECRIN MDR

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Project description:Tumor: tumor microenvironment (TME) interactions are critical for tumor progression and the composition and structure of the local extracellular matrix (ECM) are key determinants of tumor metastasis. We recently reported that activation of Wnt/beta- catenin signaling in Ewing sarcoma cells induces widespread transcriptional changes that are associated with acquisition of a metastatic tumor phenotype. Significantly, ECM protein-encoding genes were found to be enriched among Wnt/beta-catenin induced transcripts, leading us to hypothesize that activation of canonical Wnt signaling might induce changes in the Ewing sarcoma secretome. To address this hypothesis, conditioned media from Ewing sarcoma cell lines cultured in the presence or absence of Wnt3a was collected for proteomic analysis. Label-free mass spectrometry was used to identify and quantify differentially secreted proteins. We then used in silico databases to identify only proteins annotated as secreted. Comparison of the secretomes of two Ewing sarcoma cell lines revealed numerous shared proteins, as well as a degree of heterogeneity, in both basal and Wnt-stimulated conditions. Gene set enrichment analysis of secreted proteins revealed that Wnt stimulation reproducibly resulted in increased secretion of proteins involved in ECM organization, ECM receptor interactions, and collagen formation. In particular, Wnt-stimulated Ewing sarcoma cells upregulated secretion of structural collagens, as well as matricellular proteins, such as the metastasis-associated protein, tenascin C (TNC). Interrogation of published databases confirmed reproducible correlations between Wnt/beta-catenin activation and TNC and COL1A1 expression in patient tumors. In summary, this first study of the Ewing sarcoma secretome reveals that Wnt/beta-catenin activated tumor cells upregulate secretion of ECM proteins. Such Wnt/beta-catenin mediated changes are likely to impact on tumor: TME interactions that contribute to metastatic progression.

Dataset Information

Microwave Ablation or Wedge Resection for the Treatment of Lung, Sarcoma and Colorectal Lesions, ALLUME Study

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