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Efficacy and Safety of Bojungikgi-tang for Anorexia in Patients with Colorectal Cancer after Curative Resection and Chemotherapy : A single-arm, Open label, Single cencer Trial

ABSTRACT: Interventions: Drug : Participants will be administered Hanpoong Bojungikgi-tang at a dose of 75ml three times a day before or between meals for 6 weeks(42days) Primary outcome(s): The Changes of Anorexia/cachexia subscale(A/CS) of Functional Assessment of Anorexia/Cachexia Therapy(FAACT)

DISEASE(S): Diseases Of The Digestive System

PROVIDER: 2512718 | ecrin-mdr-crc |

REPOSITORIES: ECRIN MDR

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Tracing pluripotency of human early embryos and embryonic stem cells by single cell RNA-seq

Project description:Find the casual relationship between gene expression network and cellular phenotype at single cell resolution. We collected donated human pre-implatation embryos, and the embryonic stem cells derived from them, isolate individual cells, prepared single cell cDNAs, and sequenced them by HiSeq2000. Then we analyzed the expression of known RefSeq genes. We get transcriptome of 124 individual cells from human pre-implantation embryos and human embryonic stem cells by applying single cell RNA-seq technique we recently developed[1][2][3][4]. We did in-depth bioinformatic analysis to these data and found very dynamic expression of protein-coding genes. [1] Tang, F. et al. (2010a) Tracing the Derivation of Embryonic Stem Cells from the Inner Cell Mass by Single-Cell RNA-Seq Analysis. Cell Stem Cell 6, 468-478. [2] Tang, F. et al. (2010b) RNA-Seq analysis to capture the transcriptome landscape of a single cell. Nat Protocols 5, 516-535. [3] Tang, F. et al. (2009) mRNA-Seq whole-transcriptome analysis of a single cell. Nat Meth 6, 377-382. [4] Tang, F. et al. (2011) Development and applications of single-cell transcriptome analysis. Nat Meth 8, S6-S11.

2013-08-10 | E-GEOD-36552 | biostudies-arrayexpress