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FOS Immunohistochemical Staining of Colorectal Cancer and Its Adjacent Tissues

ABSTRACT: Colorectal cancer tissue sections were obtained according to the inclusion criteria. The formalin was used to immersed all cancer specimens. And tissues were cut to 5 μm thickness and placed on glass slides before staining. Endogenous peroxidase activity was inhibited and blocked by de-paraffinizing, rehydrating, and using 5% bovine serum albumin at 37ºC for 30 min. The treated sections were incubated with anti-FOS (promab 30360) at 4ºC overnight and washed three times with PBS. After that, it is required that incubation with secondary anti-peroxidation sunflower at 37ºC for 30 minutes. After washing three times again with PBS, the sections were developed in diaminobenzidine and microscopic images were made by light microscopy.

DISEASE(S): Monkeypox,Colorectal Cancer,Colorectal Neoplasms,Monkey Pox

PROVIDER: 6033 | ecrin-mdr-crc |

REPOSITORIES: ECRIN MDR

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