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MOMA Data Access Committee

ABSTRACT: Data Access Committee EGAC00001000145

PROVIDER: EGAC00001000145 | EGA |

REPOSITORIES: EGA

ACCESS DATA

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Publications

Next-generation sequencing of RNA and DNA isolated from paired fresh-frozen and formalin-fixed paraffin-embedded samples of human cancer and normal tissue.

Hedegaard Jakob J Thorsen Kasper K Lund Mette Katrine MK Hein Anne-Mette K AM Hamilton-Dutoit Stephen Jacques SJ Vang Søren S Nordentoft Iver I Birkenkamp-Demtröder Karin K Kruhøffer Mogens M Hager Henrik H Knudsen Bjarne B Andersen Claus Lindbjerg CL Sørensen Karina Dalsgaard KD Pedersen Jakob Skou JS Ørntoft Torben Falck TF Dyrskjøt Lars L

PloS one 20140530 5

Formalin-fixed, paraffin-embedded (FFPE) tissues are an invaluable resource for clinical research. However, nucleic acids extracted from FFPE tissues are fragmented and chemically modified making them challenging to use in molecular studies. We analysed 23 fresh-frozen (FF), 35 FFPE and 38 paired FF/FFPE specimens, representing six different human tissue types (bladder, prostate and colon carcinoma; liver and colon normal tissue; reactive tonsil) in order to examine the potential use of FFPE sam ...[more]

PMID: 24878701

Publication: 1/6

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