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Bioinformatics of Data Analysis (B240)

ABSTRACT: Data Access Committee EGAC00001000346

PROVIDER: EGAC00001000346 | EGA |

REPOSITORIES: EGA

ACCESS DATA

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Publications

TALEN/CRISPR-mediated engineering of a promoterless anti-viral RNAi hairpin into an endogenous miRNA locus.

Senís Elena E Mockenhaupt Stefan S Rupp Daniel D Bauer Tobias T Paramasivam Nagarajan N Knapp Bettina B Gronych Jan J Grosse Stefanie S Windisch Marc P MP Schmidt Florian F Theis Fabian J FJ Eils Roland R Lichter Peter P Schlesner Matthias M Bartenschlager Ralf R Grimm Dirk D

Nucleic acids research 20160909 1

Successful RNAi applications depend on strategies allowing robust and persistent expression of minimal gene silencing triggers without perturbing endogenous gene expression. Here, we propose a novel avenue which is integration of a promoterless shmiRNA, i.e. a shRNA embedded in a micro-RNA (miRNA) scaffold, into an engineered genomic miRNA locus. For proof-of-concept, we used TALE or CRISPR/Cas9 nucleases to site-specifically integrate an anti-hepatitis C virus (HCV) shmiRNA into the liver-speci ...[more]

PMID: 27614072

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