Project description:Genome wide DNA methylation profiling of saliva samples from male identical twins discordant for sexual orientation, age 21 to 55. The Illumina Infinium 27k Human DNA methylation Beadchip v1.2 was used to obtain DNA methylation profiles across approximately 27,000 CpGs in bisulfite converted DNA. Samples included 34 pairs of identical twins and one set of identical triplets, and 13 hybridisations were technical replicates of a selection of those samples.
Project description:Effects of transgenic Bt rice containing the Cry1Ab protein on faecal microbiome and pathology of highly inbred Wuzhishan pigs after two generations of feeding
Project description:Genome wide DNA methylation profiling of saliva samples from male identical twins discordant for sexual orientation, age 21 to 55. The Illumina Infinium 27k Human DNA methylation Beadchip v1.2 was used to obtain DNA methylation profiles across approximately 27,000 CpGs in bisulfite converted DNA. Samples included 34 pairs of identical twins and one set of identical triplets, and 13 hybridisations were technical replicates of a selection of those samples. Bisulphite converted DNA isolated from saliva of identical twin pairs were hybridised to the Illumina Infinium 27k Human Methylation Beadchip v1.2
Project description:This dataset includes transcriptome sequencing of 17 paired NAFLD-HCC samples and adjacent normal tissues. All the experiments were performed on Illumina HiSeq 2000 platform with raw reads stored in fastq format.
Project description:MKN-45 gastric cancer cells were subjected to NNMT knockdown or negative control treatment and cultured in three independent biological replicates per group. Total RNA was extracted using TRIzol reagent, and RNA integrity was confirmed with RIN > 8.0. RNA libraries were prepared using the NEBNext Ultra II RNA Library Prep Kit and sequenced on the Illumina NovaSeq 6000 platform with 150 bp paired-end reads. Differential gene expression analysis was performed to identify transcriptomic changes induced by NNMT knockdown.