Project description:Genome wide DNA methylation profiling of MZ twins whose within-pair difference in TSH were discordant. the Illumina Infinium HumanMethylation450 Bead Chip Kit (approximately 480,000 CpG sites) and the Illumina Infinium Methylation EPIC Bead Chip (approximately 900,000 CpG sites) were used to obtain DNA methylation profiles in peripheral blood samples.
Project description:Genome wide DNA methylation profiling of MZ twins whose within-pair difference in FT4 were discordant. the Illumina Infinium HumanMethylation450 Bead Chip Kit (approximately 480,000 CpG sites) and the Illumina Infinium Methylation EPIC Bead Chip (approximately 900,000 CpG sites) were used to obtain DNA methylation profiles in peripheral blood samples.
Project description:Genome-wide DNA methylation profiling of femoral bones obtained from young and old individuals. The Illumina Infinium HumanMethylation EPIC BeadChip was used to obtain DNA methylation profiles across approximately 850K CpGs from human femoral bones.
Project description:Illumina Infinium Epic Human DNA Methylation Beadchip V1.2.8 The Illumina Infinium Epic Human DNA Methylation Beadchip V1.2.8 is used to obtain DNA methylation profiles of about 850k CpG in osteoarthritis and normal cells to find differences, approximately 850k CpGs in osteoarthritis and normal cell
Project description:Using the Infinium Human DNA Methylation EPIC BeadChip array (850 K), we analyzed genome-wide DNA methylation patterns in peripheral blood leukocytes collected from 10 female patients with anorexia with primary (n= 4) or secondary (n= 6) amenorrhea and 13 healthy pubertal girls matched by age (E-MTAB-13950).
Project description:Genomic DNA was extracted from two choroid plexus carcinoma cell lines using the GeneJET genomic DNA (Thermo Fisher). DNA was quantified using the DENOVIX Fluorometer. The quality of the extracted DNA samples was assessed by the Illumina FFPE QC kit (Illumina). Bisulfite conversion of the DNA was performed using the EZ DNA methylation kit (Zymo). Bisulfite-converted DNA was hybridized to the Illumina Infinium Human Methylation EPIC array bead chip and scanned using the Illumina iScan microarray scanner (Illumina) according to the manufacturer protocol.
Project description:Genome wide DNA methylation profiling of estrogene receptor postive breast cancer cell line MCF-7, treating Ginsoenoside Rh2. The Illumina Infinium Human Methylation EPIC v1.0 B2 Bead chip was used to obtain DNA methylation profiles across approximately 850,000 CpGs. This profiling indicates that Ginsenoside Rh2 induces epigenetic and cellular changes.
Project description:Genome wide DNA methylation profiling of estrogene receptor postive breast cancer cell line MCF-7, treating Ginsoenoside Rg3. The Illumina Infinium Human Methylation EPIC v1.0 B2 Bead chip was used to obtain DNA methylation profiles across approximately 850,000 CpGs. This profiling indicates that Ginsenoside Rg3 induces epigenetic and cellular changes.
Project description:Infinium® HumanMethylation450 BeadChip and EPIC arrays were run with the aim of using the methylation profiles (n=986 in total) for sarcoma subtype classification (Paper: Lyskjær et al, 2021, DNA methylation-based profiling of bone and soft tissue tumours: a validation study of the ‘DKFZ sarcoma Classifier’ ). 500ng of DNA from fresh frozen (FT) or formalin-fixed paraffin-embedded (FFPE) tumour samples were bisulfite converted using the Zymo EZ DNA methylation Gold kit (Zymo Research Corp. Irvine, USA) before hybridisation to the Infinium HumanMethylation450 or EPIC beadchip arrays (Illumina, San Diego, CA) by UCL Genomics. All bisulfite-converted FFPE samples were restored with the Infinium FFPE DNA Restore kit (Illumina).