C169125c-f12b-40c2-b660-d7e40215f941 - samples
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ABSTRACT:
PROVIDER: EGAD00001011042 | EGA |
REPOSITORIES: EGA
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Project description:Acute viral infections can have durable functional impacts on the immune system long after recovery, but how they affect homeostatic immune states and responses to future perturbations remain poorly understood. Here we use systems immunology approaches, including longitudinal multimodal single cell analysis (surface proteins, transcriptome, and V(D)J sequences), to comparatively assess baseline immune statuses and responses to influenza vaccination in 33 healthy individuals after recovery from mild, non-hospitalized COVID-19 (mean: 151 days after diagnosis) and 40 age- and sex-matched controls who never had COVID-19. At baseline and independent of time since COVID-19, recoverees had elevated T-cell activation signatures and lower expression of innate immune genes in monocytes. COVID-19-recovered males had coordinately higher innate, influenza-specific plasmablast, and antibody responses after vaccination compared to healthy male and COVID-19-recovered females, partly because male recoverees had monocytes with higher IL-15 responses early after vaccination coupled with elevated pre-vaccination frequencies of "virtual memory" like CD8+ T-cells poised to produce more IFNg upon IL-15 stimulation. In addition, the expression of the repressed innate immune genes in monocytes increased by day 1 through day 28 post-vaccination in recoverees, thus moving towards the pre-vaccination baseline of healthy controls. In contrast, these genes decreased on day 1 and returned to the baseline by day 28 in controls. Our study reveals sex-dimorphic impacts of prior mild COVID-19 and suggests that viral infections in humans can establish new immunological set-points impacting future immune responses in an antigen-agnostic manner.
2023-01-04 | GSE206265 | GEO
Project description:Acute viral infections can have durable functional impacts on the immune system long after recovery, but how they affect homeostatic immune states and responses to future perturbations remain poorly understood. Here we use systems immunology approaches, including longitudinal multimodal single cell analysis (surface proteins, transcriptome, and V(D)J sequences), to comparatively assess baseline immune statuses and responses to influenza vaccination in 33 healthy individuals after recovery from mild, non-hospitalized COVID-19 (mean: 151 days after diagnosis) and 40 age- and sex-matched controls who never had COVID-19. At baseline and independent of time since COVID-19, recoverees had elevated T-cell activation signatures and lower expression of innate immune genes in monocytes. COVID-19-recovered males had coordinately higher innate, influenza-specific plasmablast, and antibody responses after vaccination compared to healthy male and COVID-19-recovered females, partly because male recoverees had monocytes with higher IL-15 responses early after vaccination coupled with elevated pre-vaccination frequencies of "virtual memory" like CD8+ T-cells poised to produce more IFNg upon IL-15 stimulation. In addition, the expression of the repressed innate immune genes in monocytes increased by day 1 through day 28 post-vaccination in recoverees, thus moving towards the pre-vaccination baseline of healthy controls. In contrast, these genes decreased on day 1 and returned to the baseline by day 28 in controls. Our study reveals sex-dimorphic impacts of prior mild COVID-19 and suggests that viral infections in humans can establish new immunological set-points impacting future immune responses in an antigen-agnostic manner.
2022-03-09 | GSE194378 | GEO
Project description:We report here a double-blinded pilot study of seasonal influenza vaccination, where half of the participants received daily metformin administration. Global metabolomics was measured in the plasma samples at six timepoints. Metformin signatures were successfully identified in the metabolomics data. Statistically significant metabolite features were found both for the vaccination effect and for the drug-vaccine interactions.
2023-01-04 | ST002432 | MetabolomicsWorkbench
Project description:Changes in the urinary proteome before and after quadrivalent influenza vaccineQIV and COVID-19 vaccination
2022-06-28 | PXD034949 | iProX
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Project description:Expression data of BL2 Burkitt Lymphoma cell line (controls and samples treated with different B cell specific stimuli) BL2 cells were cultured in RPMI and stimulated with the respective agents (rhCD40L, rhIL21, rhBAFF, LPS and antiIgM F(ab) fragments) for indicated timepoints. Cells were harvested and total RNA was isolated for gene expression profiling. The experiments were conducted in 2-3 biological replicates.
2013-02-01 | E-GEOD-42660 | biostudies-arrayexpress
Project description:The purpose of the study was to assess the patterns of global gene expression in peripheral blood cells before and at three time points after the administration of a trivalent influenza vaccine in human male subjects, and to relate these to the antibody response to the vaccine. The antibody titer data for these subjects is provided as a supplemental file. 119 healthy male human volunteers ages 19 - 41 years were recruited in the study. Whole-blood RNA samples were collected in PaxGene tubes before and at three time points after administration of a trivalent influenza vaccine. Baseline samples (before vaccination) are labeled as day 0. The three additional time points are days 1, 3, and 14 after vaccination. A total of 431 arrays, corresponding to 116 individuals, passed quality control and are included in this data set. These are distributed as follows: Day 0: 111 Samples Day 1: 110 Samples Day 3: 101 Samples Day 14: 109 Samples
2013-06-18 | E-GEOD-48018 | biostudies-arrayexpress
Project description:Systems biology approach to examine effects of seasonal flu vaccination in adults of different ages on gene expression, cytokine stimulation and serum cytokines with parameters such as immune senescence to uncover new markers and mechanisms behind failure of immune function in many older people. Total RNA was extracted from whole-blood lysates obtained from individuals immediately prior to vaccination against seasonal influenza, followed by depletion of globin messenger RNA. The goal was to evaluate potential gene expression signatures at Day 0 associated with immune senescence.
2014-07-20 | E-GEOD-58898 | biostudies-arrayexpress
Project description:Systems biology approach to examine effects of seasonal flu vaccination in adults of different ages on gene expression, cytokine stimulation and serum cytokines with parameters such as immune senescence to uncover new markers and mechanisms behind failure of immune function in many older people. Total RNA was extracted from whole-blood lysates obtained from individuals immediately prior to vaccination against seasonal influenza, followed by depletion of globin messenger RNA. The goal was to evaluate potential gene expression signatures at Day 0 associated with immune senescence.
2014-07-20 | E-GEOD-58943 | biostudies-arrayexpress
Project description:To assess changes in immune gene expression following HIV-env vaccination through 3 delivery systems, rhesus macaques were vaccinated with SOSIP trimer with adjuvants using either oral, intraepithelial delivery through a syringe (IEP), a needle free pressurized oral delivery (NF), or an intramuscular injection in the right deltoid (IM). Submandibular lymph nodes were biopsied and stored in RPMI supplemented with 10% fetal bovine serum. RNA was extracted using Macherey Nagel nucleospin columns. RNA from both timepoints was then used for gene expression analysis using the Nanostring nCounter platform.
2020-07-01 | GSE148124 | GEO