Project description:The goal of the current study was to examine the extent to which TGF-beta signaling contributes to insulin-induced transcriptional responses in endothelial cells (HUVECs). HUVECs cells were treated with or without insulin in the absence or presence of SB431542, a TGF-beta receptor kinase inhibitor. Cells were sequenced using Hiseq 2000 (Illumina) as paired-end 2x100 base reads.
Project description:Genome wide DNA methylation profiling of monocytes from insulin sensitive and insulin resistant HIV-infected patients. The Illumina Infinium Human Methylation 450k Beadchip was used to obtain DNA methylation profiling across 450,000+ CpGs. Samples included monocytes from 37 HIV-infected individuals, of which 14 were insulin resistant and 23 were insulin sensitive, monocytes from 9 HIV-seronegative individuals, of which 4 were insulin sensitive and 5 insulin resistant, and FACS monocyte subsets (classical, intermediate, and non-classical monocytes) from two healthy donors.