Population whole-genome bisulfite sequencing across two tissues highlights environment as principal source of human methylome variation

ABSTRACT: The study includes whole-genome bisulfite sequencing (WGBS) on 34 adipose (7 MZ pairs, 6 DZ pairs and 8 singletons) and 27 blood (7 MZ pairs, 6 DZ pairs and 1 singleton) DNA samples derived from a total of 43 female twins belonging to the MuTHER/TwinsUK cohort. We generated 11.5 billion 100bp paired-end reads covering 2.3 Tera-basepairs (Tbp) of sequence using the Illumina HiSeq2000 or 2500 systems. Applying standard alignment methods and filters we obtain a mean genome coverage of 6.3-fold (range: 1.0- to 12.9-fold) for adipose and 8.7-fold (range: 0.7- to 29.0-fold) for blood.

PROVIDER: EGAS00001001569 | EGA |

REPOSITORIES: EGA