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Single-cell RNA sequencing on 5063 single T cells isolated from peripheral blood, tumour and adjacent normal tissues from six hepatocellular carcinoma patients.

ABSTRACT: Single-cell RNA sequencing on 5063 single T cells isolated from peripheral blood, tumour and adjacent normal tissues from six hepatocellular carcinoma patients. The transcriptional properties of these individual cells, coupled with the assembled T cell receptor (TCR) sequences, were used to delineate the molecular subtypes, lineage, and functional properties of each T cell group. For five HCC patient, the exome-seq data of matched normal/tumor and bulk RNA-seq data of tumor are also available.

PROVIDER: EGAS00001002072 | EGA |

REPOSITORIES: EGA

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Landscape of Infiltrating T Cells in Liver Cancer Revealed by Single-Cell Sequencing.

Zheng Chunhong C Zheng Liangtao L Yoo Jae-Kwang JK Guo Huahu H Zhang Yuanyuan Y Guo Xinyi X Kang Boxi B Hu Ruozhen R Huang Julie Y JY Zhang Qiming Q Liu Zhouzerui Z Dong Minghui M Hu Xueda X Ouyang Wenjun W Peng Jirun J Zhang Zemin Z

Cell 20170601 7

Systematic interrogation of tumor-infiltrating lymphocytes is key to the development of immunotherapies and the prediction of their clinical responses in cancers. Here, we perform deep single-cell RNA sequencing on 5,063 single T cells isolated from peripheral blood, tumor, and adjacent normal tissues from six hepatocellular carcinoma patients. The transcriptional profiles of these individual cells, coupled with assembled T cell receptor (TCR) sequences, enable us to identify 11 T cell subsets b ...[more]

PMID: 28622514

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