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Transcriptome sequencing of myelodysplasia

ABSTRACT: Transcriptome sequencing was performed on 214 patients with myelodysplasia in this study. RNA was obtained from bone marrow CD34+ cells (n=100) and/or bone marrow mononuclear cells (n=165). Transcriptome sequencing was performed for both cell fractions in 51 patients. We also studied bone marrow CD34+ cells and bone marrow mononuclear cells obtained from three healthy adults each.

PROVIDER: EGAS00001002346 | EGA |

REPOSITORIES: EGA

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Gene expression and risk of leukemic transformation in myelodysplasia.

Shiozawa Yusuke Y Malcovati Luca L Gallì Anna A Pellagatti Andrea A Karimi Mohsen M Sato-Otsubo Aiko A Sato Yusuke Y Suzuki Hiromichi H Suzuki Hiromichi H Yoshizato Tetsuichi T Yoshida Kenichi K Yoshida Kenichi K Shiraishi Yuichi Y Chiba Kenichi K Makishima Hideki H Boultwood Jacqueline J Hellström-Lindberg Eva E Miyano Satoru S Cazzola Mario M Ogawa Seishi S

Blood 20171102 24

Myelodysplastic syndromes (MDSs) are a heterogeneous group of clonal hematopoietic disorders with a highly variable prognosis. To identify a gene expression-based classification of myelodysplasia with biological and clinical relevance, we performed a comprehensive transcriptomic analysis of myeloid neoplasms with dysplasia using transcriptome sequencing. Unsupervised clustering of gene expression data of bone marrow CD34<sup>+</sup> cells from 100 patients identified 2 subgroups. The first subty ...[more]

PMID: 29097382

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ena-DATASET-CGP-UT-04-01-2018-02:12:18:566-617 - samples

Project description:Transcriptome sequencing was performed on 214 patients with myelodysplasia in this study. RNA was obtained from bone marrow CD34+ cells (n=100) and/or bone marrow mononuclear cells (n=165). Transcriptome sequencing was performed for both cell fractions in 51 patients. A total of 211 patients were genotyped by targeted deep sequencing. We also studied bone marrow CD34+ cells and bone marrow mononuclear cells obtained from three healthy adults each.

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