Dataset Information


Tissue-specific cell-free DNA degradation quantifies circulating tumor DNA burden

ABSTRACT: Profiling of circulating tumor DNA (ctDNA) may offer a non-invasive approach to monitor disease progression. Here, we developed a quantitative method, exploiting local tissue-specific cell-free DNA (cfDNA) degradation patterns, that accurately estimates ctDNA burden independent of genomic aberrations. Nucleosome-dependent cfDNA degradation at promoters and first exon-intron junctions was strongly associated with differential transcriptional activity in tumors and blood. A quantitative model, based on just 6 regulatory regions, could accurately predict ctDNA levels in colorectal cancer patients. Strikingly, a model restricted to blood-specific regulatory regions could predict ctDNA levels across both colorectal and breast cancer patients.

ORGANISM(S): Homo sapiens  

PROVIDER: EGAS00001004657 | EGA |


Similar Datasets

1000-01-01 | S-EPMC4453461 | BioStudies
2020-01-01 | S-EPMC7340299 | BioStudies
2020-01-01 | S-EPMC7059766 | BioStudies
2019-01-01 | S-EPMC6398498 | BioStudies
2019-01-01 | S-EPMC6687711 | BioStudies
2019-01-01 | S-EPMC6521186 | BioStudies
1000-01-01 | S-EPMC6361573 | BioStudies
2019-01-01 | S-EPMC6771167 | BioStudies
2018-01-01 | S-EPMC5828212 | BioStudies
1000-01-01 | S-EPMC6197164 | BioStudies