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Oxford Nanopore RNA sequencing for HLA typing

ABSTRACT: A targeted RNA-based method for typing of 12 classical HLA genes using Oxford Nanopore sequencing. In the method, were enriched HLA genes from cDNA of 50 individuals using gene-specific reverse primers. The library molecules were then barcoded, pooled into 2 separate gene pools, and sequenced on MinION R9.4 SpotON flow cells.

PROVIDER: EGAS00001004918 | EGA |

REPOSITORIES: EGA

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