Project description:Growth and transcriptional profiles of the barophilic methanarchaeon Methanocaldococcus jannaschii were studied at temperatures up to 98C and pressures up to 500 atm. Application of 500 atm of hyperbaric pressure shifted the optimal growth temperature upwards, and heat shock from 88C to 98C at 500 atm resulted in termination of growth. Pressure shock of M. jannaschii from 7.8 to 500 atm over 15-min, the first pressure upshift reported for a barophile, did not accelerate growth. Transcriptional profiles indicated a similar pressure response under growth and heat shock at 500 atm and pressure shock to 500 atm suggesting that the commonly affected genes are important for high-pressure adaptation. Factorial microarray design allowed de-convolution of the interacting effect of elevated pressure and heat shock on expression profiles, thus suggesting genes that may contribute to the organism’s survival in the turbulent in situ conditions of deep-sea hydrothermal vents. Keywords: stress response, time course, high pressure, heat shock, pressure shock

2007-04-05 | GSE4620 | GEO

BGISEQ-500 WGS

Project description:The performance of BGISEQ-500 for whole genome sequencing.

| PRJEB19427 | ENA

BGISEQ-500 WES

Project description:The performance of BGISEQ-500 for whole exome sequencing

| PRJEB19426 | ENA

Solid digest

Project description:Solid digest Raw sequence reads

| PRJNA760953 | ENA

BGISEQ-500 RNA-Seq

Project description:The performance of BGISEQ-500 for RNA-Seq