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Genetic measurement of memory B-cell recall using antibody repertoire sequencing

ABSTRACT:

We developed an improved high throughput sequencing approach to measure the quantities and sequences of the repertoire of antibody heavy chain RNA in a blood sample. Using this approach we analyzed the antibody repertoire in response to yearly vaccinations with influenza vaccines TIV and LAIV in healthy adults in two subsequent years. We determined vaccine response patterns specific to LAIV and TIV and found antibody sequences that were shared between two samples of the same individuals following influenza vaccination in subsequent years, thereby providing a genetic measurement of B-cell memory recall.

PROVIDER: phs000656.v1.p1 | EGA |

REPOSITORIES: EGA

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Genetic measurement of memory B-cell recall using antibody repertoire sequencing.

Vollmers Christopher C Sit Rene V RV Weinstein Joshua A JA Dekker Cornelia L CL Quake Stephen R SR

Proceedings of the National Academy of Sciences of the United States of America 20130729 33

Annual influenza vaccinations aim to protect against seasonal infections, and vaccine strain compositions are updated every year. This protection is based on antibodies that are produced by either newly activated or memory B cells recalled from previous encounters with influenza vaccination or infection. The extent to which the B-cell repertoire responds to vaccination and recalls antibodies has so far not been analyzed at a genetic level--which is to say, at the level of antibody sequences. Her ...[more]

PMID: 23898164

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