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Genome-wide association across Saccharomyces cerevisiae strains reveals substantial variation in underlying gene requirements for toxin tolerance.

ABSTRACT: We exploited natural diversity in 165 Saccharomyces cerevisiae strains isolated from diverse geographical and ecological niches, to identify mechanisms of hydrolysate-toxin tolerance. We performed genome-wide association (GWA) analysis to identify genetic variants underlying toxin tolerance, and gene knockouts and allele-swap experiments to validate the involvement of implicated genes. In the process of this work, we uncovered a surprising difference in genetic architecture depending on strain background: in all but one case, knockout of implicated genes had a significant effect on toxin tolerance in one strain, but no effect in another strain.

INSTRUMENT(S): Illumina HiSeq 2000

ORGANISM(S): Saccharomyces Cerevisiae S288c

SUBMITTER: University of Wisconsin-Madison

PROVIDER: PRJEB24747 | EVA | 2018-02-02

REPOSITORIES: EVA

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