Scaffolding of Ambystoma mexicanum chromosomes
Ontology highlight
ABSTRACT:
INSTRUMENT(S): Illumina HiSeq 2500
ORGANISM(S): Ambystoma 'unisexual Hybrid'
SUBMITTER: University of Kentucky
PROVIDER: PRJEB30506 | EVA | 2019-01-14
REPOSITORIES: EVA
Similar Datasets
Project description:Genetic recombination generates novel trait combinations, and understanding how recombination is distributed across the genome is key to modern genetics. The PRDM9 protein defines recombination hotspots; however, megabase-scale recombination patterning is independent of PRDM9. The single round of DNA replication, which precedes recombination in meiosis, may establish these patterns; therefore, we devised an approach to study meiotic replication that includes robust and sensitive mapping of replication origins. We find that meiotic DNA replication is distinct; reduced origin firing slows replication in meiosis, and a distinctive replication pattern in human males underlies the subtelomeric increase in recombination. We detected a robust correlation between replication and both contemporary and historical recombination and found that replication origin density coupled with chromosome size determines the recombination potential of individual chromosomes. Our findings and methods have implications for understanding the mechanisms underlying DNA replication, genetic recombination, and the landscape of mammalian germline variation.
2021-07-02 | GSE148327 | GEO
Project description:Hair shaft is one of the most common biological evidences found at crime scene. Due to the low amount and high degradation of nuclear DNA in hair shaft, it is difficult to achieve individual identification through routine method. The proteins in the hair shaft are stable and contain genetic polymorphisms in the form of single amino acid polymorphisms (SAPs), translated from non-synonymous single nucleotide polymorphisms (nsSNPs) in the genome.
2022-06-22 | PXD034814 |
Project description:Large-scale chromosome structure and spatial nuclear arrangement have been linked to control of gene expression and DNA replication and repair. Genomic techniques based on chromosome conformation capture assess contacts for millions of loci simultaneously, but do so by averaging chromosome conformations from millions of nuclei. Here we introduce single cell Hi-C, combined with genome-wide statistical analysis and structural modeling of single copy X chromosomes, to show that individual chromosomes maintain domain organisation at the megabase scale, but show variable cell-to-cell chromosome territory structures at larger scales. Despite this structural stochasticity, localisation of active gene domains to boundaries of territories is a hallmark of chromosomal conformation, affecting most domains in most nuclei. Single cell Hi-C data bridge current gaps between genomics and microscopy studies of chromosomes, demonstrating how modular organisation underlies dynamic chromosome structure, and how this structure is probabilistically linked with genome activity patterns.
2013-09-26 | GSE48262 | GEO
Project description:Most proteogenomic approaches for mapping single amino acid polymorphisms (SAPs) require construction of a sample-specific database containing protein variants predicted from the next-generation sequencing (NGS) data. We present a new strategy for direct SAP detection without relying on NGS data. Among the 348 putative SAP peptides identified in an industrial yeast strain, 85.6% of SAP sites were validated by genomic sequencing.
2018-04-18 | PXD003101 | Pride
Project description:We designed a novel tiling array, AtMap1, for genomic deletion mapping. The AtMap1 is a 60-mer oligonucleotide microarray consisting of 42,497 data probes designed from the genomic sequence of Arabidopsis thaliana Col-0. The data probes are almost evenly distributed over the genome except around centromeres. The average probe interval is 2.8 kb. The performance of the AtMap1 array was assessed using the mag2-2 mutant, which is a well-studied deletion mutant. Eight of the probes showed 3-fold lower signals in mag2-2 than Col-0. Seven of these probes were located in one region on chromosome 3. We treated these adjacent probes as one deletion. This deletion included previously reported deleted region. The other probe was located near the end of chromosome 4. A deletion around the probe was confirmed by PCR. This deletion was newly identified in this study. Thus, we concluded that the AtMap1 array was sufficiently sensitive to identify a deletion without a priori knowledge. From an analysis using the result of hybridization of Ler and previously reported polymorphism data, we found a tendency that the signal decrease depended on the overlap size of sequence polymorphisms. Mutation mapping is time-consuming, laborious and costly. The AtMap1 array removes the difficulties. The AtMap1 array is useful especially for mutations for which linkage-based mapping is difficult, e.g., mutations whose phenotype is highly variable among ecotypes, mutations whose phenotype is difficult to measure and revertant mutations. Keywords: comparative genomic hybridization
2007-09-01 | GSE8120 | GEO
Project description:Epigenomic mapping of DNA methylation polymorphisms segregating within an epigenetic Recombinant Inbred Line population resultant from crossing the wild-type Col-0 and DNA maintenance methyltransferase mutant, met1-3, parental lines. Each line within the epiRIL population was self-fertilized for 7 generations, 10 individual F7 plants were then bulk harvested to create the F8 generation. Here, data files for three DNA methylation profiled epiRIL lines (epi01, epi12, epi28) are deposited. The DNA methylation polymorphisms were determined using the BiMP method with the Affymetrix Arabidopsis Tiling 1.0R array. The main objective for these samples was to create an epigenomic map to analyze the inheritance of DNA methylation polymorphisms within these three epiRIL entries. Keywords: Bisulfite Methylation Profiling (BiMP) by genome tiling array
2009-04-23 | GSE13438 | GEO
Project description:Genomic integrity requires faithful chromosome duplication. Origins of replication are the genomic sites where DNA replication initiates in every cell cycle. There are multiple origins scattered throughout the eukaryotic genome whose genome-wide identification has been a hard challenge, especially in multicellular organisms. Thus, very little is known on the distinctive features of origins in terms of DNA sequence and chromatin context at a genomic scale. Here we have profiled origins in Arabidopsis thaliana by high-throughput sequencing of purified nascent DNA strands. We have identified 1543 replication origins, which were uniformly distributed across the Arabidopsis genome and enriched in binding signals of two replication initiation proteins, CDC6 and ORC1. We have also analyzed novel epigenome maps of various histone modifications and found links between origins and epigenetic signatures, which differ from or have not been reported for other eukaryotic systems. Arabidopsis origins tend to be embedded in G+C-rich regions within the 5M-bM-^@M-^Y half of genes, enriched in histone H2A.Z, H3K4me2/3 and acetylated H4, and depleted of H3K4me1 and H3K9me2. Our data establish the basis for the understanding of the epigenetic specification of origins of replication in Arabidopsis and have implications for the mechanisms of origin specification in other eukaryotes. This SuperSeries is composed of the following subset Series: GSE21781: Mapping origins of replication in Arabidopsis thaliana: Examination of BrdU labelled DNA and unlabelled DNA in one cell type GSE21827: Mapping origins of replication in Arabidopsis thaliana: H3K4ac ChIP vs. unmodified H3 ChIP Refer to individual Series
2011-02-06 | E-GEOD-21828 | biostudies-arrayexpress
Project description:Purpose: Recent development in high-throughput sequencing techniques (RNA-seq) has enabled large scale analysis of genetic variations and gene expression in different tissues and species, but gene expression patterns and genetic variations in livestock have not yet been well characterized. In this study we have used high-throughput transcriptomic sequencing of the Finnish Yorkshire to identify gene expression patterns within the breed in the testis and oviduct. Methods: The Solid 4 reads were mapped against the pig genome build 10.2 using the colorspace alignment tool provided by Applied Biosystems and distributed with the instrument (LifeScope v2.1). Reads associated with ribosomal RNA, transfer RNA, repeats and other uninformative reads were filtered out during the process as well as reads with more than 10 potential alignments. After alignment to the reference genome, low mapping quality reads were discarded (mapQV(<10)) and unique reads were associated with known genes based on UCSC annotations, and the number of reads aligned within each gene was counted. FPKM values were calculated for normalization of the data to remove variation between samples caused by non-biological reasons using the Cufflinks software v2.0.2. Results: The analysis of gene expression differences between the testis and oviduct highlighted 1,234 genes up-regulated in the testis and 1,501 in the oviduct. Conclusions: The RNA-seq technology used in this study provides novel information about transcript expression and differential gene expression in the testis and oviduct. The produced data will assist in the identification of candidate genes based on association mapping results within the pig population and provides insights into the expression of genes in the two reproductive organs studied. Testis and oviduct mRNA profiles of adult WT and immotile short tail sperm (ISTS) affected Finnish Large White pigs were generated by deep sequencing using Solid 4 platform.
2015-08-06 | E-GEOD-59149 | biostudies-arrayexpress
Project description:Cytosine methylation in the genome of Drosophila melanogaster has been elusive and controversial: methylcytosine has been detected at very low levels in early embryos, but the genomic location and function of methylation has not been established. We have mapped cytosine methylation genomewide in Stage 5 Drosophila embryo DNA by combining immuno-enrichment for 5-methylcytosine, bisulfite conversion, and deep sequencing. Unlike methylation patterns observed in other eukaryotic species, methylation in Drosophila is punctate and highly strand-asymmetrical; we confirmed this by direct PCR amplification and sequencing of bisulfite-converted DNA. Despite the locally asymmetric nature of methylation, large-scale patterns of methylation are symmetric. Methylated regions make up ~1% of the genome, and within these regions methylation of individual cytosines averages 2-10%. Methylation is concentrated in specific 5-base sequence motifs that are CA- and CT-rich but depleted of guanine. It is depleted from promoters, coding sequences, and most retrotransposons, and enriched in introns and in certain simple sequence repeats containing the commonly methylated motifs. Comparison with available gene expression data indicates that methylation in a gene is associated with lower expression; the X chromosome, which is subject to gene dosage compensation, is more densely methylated than the autosomes. This study firmly establishes the presence of cytosine methylation in Drosophila; the temporal overlap of methylation with the maternal-zygotic transition raises the possibility that methylation participates in the transition to zygotic gene expression.
2014-02-18 | GSE34425 | GEO
Project description:Albeit with low content, in genome DNA, the 5-formyluracil has been an important modification. The 5-formyluracil was found to be widely distributed amongst living bodies (prokaryotes and mammalian cells). Capable of binding to proteins to prevent the gene expressions, the 5-formyluracil could also induce mismatch in the DNA. In certain cancerous tissues, the content of 5-formyluracil was found to be higher than the normal tissues adjacent to the tumor. Nonetheless, the lack of a higher resolution mapping technique, has been hampering the studies of 5-formyluracil. Hence, we adopted the single-base resolution analysis method to profile 5-formyluracil at the genome scale. We analyzed the distribution of 5-formyluracil in the genome. This technique enabled us to have a better understanding of 5-formyluracil.
2022-12-01 | GSE164138 | GEO