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AmelHap: Leveraging drone whole-genome sequence data to create a honey bee HapMap

ABSTRACT: Honey bee, Apis mellifera, drones are typically haploid, developing from an unfertilized egg, inheriting only their queen?s alleles and none from the many drones she mated with. Thus the ordered combination or `phase? of alleles is known, making drones a valuable haplotype resource. We collated whole-genome sequence data for 1,407 drones, including 45 newly sequenced Scottish drones, collectively representing 19 countries, 8 subspecies and various hybrids. Following alignment to Amel_HAv3.1, variant calling and quality filtering, we retained 17.4 M high quality variants across 1,328 samples with a genotyping rate of 98.7%. We demonstrate the utility of this haplotype resource, AmelHap, for genotype imputation, returning >95% concordance when up to 64% of data is missing in haploids and up to 20% of data is missing in diploids. AmelHap will serve as a useful resource for the community for imputation from low-depth sequencing or SNP chip data, accurate phasing of diploids for association studies, and as a comprehensive reference panel for population genetic and evolutionary analyses.

INSTRUMENT(S): Illumina

ORGANISM(S): Apis Mellifera

SUBMITTER: Roslin Institute

PROVIDER: PRJEB59912 | EVA | 2023-02-16

REPOSITORIES: EVA

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Dataset's files

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			Action	DRS
	AmelHap_1.1.1.f4.accessioned.vcf.gz	Vcf
	AmelHap_1.1.1.f4.accessioned.vcf.gz.csi	Vcf
	AmelHap_1.1.1.f4.vcf.csi	Other
	AmelHap_1.1.1.f4.vcf.gz	Vcf

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