Dataset Information

Exploring sequence diversity in Napier grass to fast-track forage breeding in Africa

ABSTRACT: The overarching goal of the proposal is to provide livestock farmers in the sub-Sahara geographic region with better varieties of Napier grass (Cenchrus purpureus) that will support the efficient, sustainable and resilient livestock systems. To ensure the timely development of forage cultivars adapted to specific agroclimatic conditions in a changing environment, we propose to shorten the breeding cycle using state-of-the-art analysis of the natural genetic variation in the species. Specifically, we propose to capture the maximal range of polymorphisms by whole-genome sequencing of hundreds of accessions of Napier grass with the project focus on the identification of molecular markers associated with drought tolerance, crude protein content, water soluble carbohydrates, self-incompatibility, disease resistance and frost tolerance traits. The generated data will be essential to spearhead and expand phenotype-genotype association studies by the scientific community at all levels.

INSTRUMENT(S): Illumina NovaSeq 6000

ORGANISM(S): Cenchrus Purpureus

SUBMITTER: ILRI

PROVIDER: PRJEB88573 | EVA | 2025-04-18

REPOSITORIES: EVA

ACCESS DATA

Dataset's files

Source:

			Action	DRS
	all.snps_filtered.accessioned.vcf.gz	Vcf
	all.snps_filtered.accessioned.vcf.gz.csi	Vcf
	all.snps_filtered.vcf.gz.biallelic_MAF01_MAXMISS050_thin50bp.renamed.vcf.csi	Vcf
	all.snps_filtered.vcf.gz.biallelic_MAF01_MAXMISS050_thin50bp.renamed.vcf.gz	Vcf

Items per page:

1 - 4 of 4

Similar Datasets

Project description:External application of acetic acid has been recently reported to enhance the survival to drought in plants such as Arabidopsis, rapeseed, maize, rice and wheat, but the effects of acetic acid application on increased drought tolerance in woody plants such as a tropical crop “cassava” remain elusive. A molecular understanding of acetic acid-induced drought avoidance in cassava will contribute to the development of technology that can be used to enhance drought tolerance without resorting to transgenic technology or advancements in cassava cultivation. In the present study, morphological, physiological and molecular responses to drought were analyzed in cassava after the treatment with acetic acid. Results indicated that the acetic acid-treated cassava plants had a higher level of drought avoidance than water-treated, control plants. Specifically, higher leaf relative water content, and chlorophyll and carotenoid levels were observed as soils dried out during the drought treatment. Leaf temperatures in acetic acid-treated cassava plants were higher relative to leaves on plants pretreated with water and the increase of ABA content was observed in leaves of acetic acid-treated plants, suggesting that stomatal conductance and the transpiration rate in leaves of acetic acid-treated plants decreased to maintain relative water contents and avoid drought. Transcriptome analysis revealed that the acetic acid treatment increased the expression of ABA signaling-related genes, such as OPEN STOMATA 1　(OST1) and protein phosphatase 2C; as well as drought response and tolerance-related genes, such as outer membrane tryptophan-rich sensory protein (TSPO), and heat shock proteins. Collectively, the external application of acetic acid enhances drought avoidance in cassava through the upregulation of ABA signaling pathway genes and several stress response- and tolerance-related genes. These data support the idea that adjustments of the acetic acid application to plants is useful to enhance drought tolerance in order to minimize the growth inhibition in the agricultural field.

Dataset Information

Exploring sequence diversity in Napier grass to fast-track forage breeding in Africa

Dataset's files

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets