Project description:The traditional Chinese medical formulas have been in clinical use for thousands of years and their therapeutic effects were documented in ancient Chinese pharmacopoeias. High-throughput biological analysis on cell line models has been demonstrated as a useful alternative to elucidate intricate molecular mechanisms associated with drug actions in a number of pharmacogenomic studies. This microarray study is aimed to provide a reliable and feasible means to explore the healing philosophy of Chinese herbalism. Keywords: Pharmacogenetics
Project description:We perform the first two-stage GWAS on TB in the Chinese population. We examined 900,015 genetic variants in 1,008 TB patients using the HumanOmniZhongHua-8 v1.1 BeadChip.
Project description:Chinese and Philippine strains of the blood fluke Schistosoma japonicum present clear and distinctive phenotypes in areas of fecundity, pathology, drug sensitivity and immunology. Despite these differences large scale sequencing efforts have focused solely on Chinese mainland strain of the parasite. We have undertaken a comparative genomic hybridisation (CGH) approach to highlight some of the structural differences in the genome of two of the major geographical isolates of S. japonicum. We identified seven distinct regions of the S. japonicum genome that present differential CGH between Chinese and Philippine strains of the blood fluke Schistosoma japonicum, representing either deletion or duplication regions in the Philippine strain. Within these regions, genes that may be related to phenotypical differences are identified and discussed.
Project description:Our study is the first transcriptome profiling of Chinese NPC patients. These results provide both molecular basis and therapeutic opportunities for Chinese NPC patients.
Project description:Our study is the first transcriptome profiling of Chinese NPC patients. These results provide both molecular basis and therapeutic opportunities for Chinese NPC patients. mRNA profiles of 42 Chinese Nasopharyngeal Carcinoma patients and 4 non-NPC tissues by Illumina Hiseq 2000.
Project description:Chinese yam (Dioscorea opposita Thumb.) is a highly nutritional perennial herbaceous rhizome. N-glycoforms from Chinese yam glycoprotein were analyzed, and the major glycoprotein in yam be identificated.
Project description:Chinese and Philippine strains of the blood fluke Schistosoma japonicum present clear and distinctive phenotypes in areas of fecundity, pathology, drug sensitivity and immunology. Despite these differences large scale sequencing efforts have focused solely on Chinese mainland strain of the parasite. We have undertaken a comparative genomic hybridisation (CGH) approach to highlight some of the structural differences in the genome of two of the major geographical isolates of S. japonicum. We identified seven distinct regions of the S. japonicum genome that present differential CGH between Chinese and Philippine strains of the blood fluke Schistosoma japonicum, representing either deletion or duplication regions in the Philippine strain. Within these regions, genes that may be related to phenotypical differences are identified and discussed. Genomic DNA was isolated from adult (7 week post cercarial challenge) Schistosoma japonicum Chinese and Philippine isolates and separate maleand femalesamples comparatively hybridised on an Agilent customn designed oligo microarray.
Project description:Leprosy is a chronic granulomatous disease caused by infection with Mycobacterium leprae. Genetic association studies indicated that leprosy risk is strongly associated with variation within the major histocompatibility complex (MHC) region, but the full number of variants in this region has yet to be elucidated. To identify further susceptibility loci or loss of function variants for this disease, we performed fine-mapping analysis of the MHC region using a Han Chinese reference panel (n= 10,689 patients, 29,948 genetic markers) in the data sets from our previous leprosy studies. Then, a fixed-effect meta-analysis was carried out separately for Chinese (case=2,901, control=3,801) and North Chinese (case=1,983, control=2,635) participants. The meta-analysis of Chinese participants identified 10 HLA-type or amino acid variants with lower than the genome-wide significant susceptibility signal. Next, gene-by-gene step-wise conditional analysis was performed in the combined dataset of these cohorts. Finally, we identified four new independent susceptibility loci (HLA-DQA1, HLA-C, rs3129063, and rs58327373) and confirmed one previously reported locus (HLA-DRB1) that significantly associated with leprosy in the Chinese Han population. Thus the results of this study increase knowledge about leprosy risk variants and illustrate the value of HLA imputation for fine mapping of causal variants in the MHC.