Dataset Information
Final model of TryS
Ontology highlight
ABSTRACT:
SUBMITTER: Jurgen Haanstra
PROVIDER: 108 | FAIRDOMHub | 2014-07-31
REPOSITORIES: FAIRDOMHub
Publications
Dissecting the catalytic mechanism of Trypanosoma brucei trypanothione synthetase by kinetic analysis and computational modeling.
The Journal of biological chemistry 20130628 33
In pathogenic trypanosomes, trypanothione synthetase (TryS) catalyzes the synthesis of both glutathionylspermidine (Gsp) and trypanothione (bis(glutathionyl)spermidine (T(SH)2)). Here we present a thorough kinetic analysis of Trypanosoma brucei TryS in a newly developed phosphate buffer system at pH 7.0 and 37 °C, mimicking the physiological environment of the enzyme in the cytosol of bloodstream parasites. Under these conditions, TryS displays Km values for GSH, ATP, spermidine, and Gsp of 34, ...[more]
