Project description:Stimulated by our 2015 Current Biology paper , Zambon et al. reinvestigated how three myosin isoforms participate in the formation and constriction of the contractile ring in fission yeast. Our paper presented evidence that these myosin isoforms have distinct roles: "Conventional myosin-II Myo2 is crucial to ring assembly, unconventional myosin-II Myp2 is most important for ring constriction, and type V myosin Myo51 aids the other two myosins." Zambon et al. used different markers to reexamine the contributions of the three myosins to cytokinesis and concluded "that Myo2p is the major motor involved in ring contraction in S. pombe." Here, we show that most of the differences observed by Zambon et al. can be attributed to their use of the Rlc1p-3GFP marker, which genetically interacts with myo2-E1.
Project description:We would like to respond to Brosch et al. regarding our manuscript "Expression of the Splicing Factor Gene SFRS10 Is Reduced in Human Obesity and Contributes to Enhanced Lipogenesis" (Pihlajamäki et al., 2011b). Brosch performed RT-PCR in liver samples from 13 lean and 34 obese individuals, finding no differences in SFRS10 or LPIN1 expression. We wish to address points raised by Brosch, including experimental strategy and analysis of human SFRS10 expression.
Project description:A diverse body of research has demonstrated that people update their beliefs to a greater extent when receiving good news compared to bad news. Recently, a paper by Shah et al. claimed that this asymmetry does not exist. Here we carefully examine the experiments and simulations described in Shah et al. and follow their analytic approach on our data sets. After correcting for confounds we identify in the experiments of Shah et al., an optimistic update bias for positive life events is revealed. Contrary to claims made by Shah et al., we observe that participants update their beliefs in a more Bayesian manner after receiving good news than bad. Finally, we show that the parameters Shah et al. pre-selected for simulations are at odds with participants' data, making these simulations irrelevant to the question asked. Together this report makes a strong case for a true optimistic asymmetry in belief updating.
Project description:A review and reanalysis of geological, molecular, and paleontological data led O'Dea et al. (1) to propose (i) that reports by Montes et al. (2) and Bacon et al. (3) regarding a middle Miocene closure of the Central American Seaway (CAS) are unsupported, and (ii) a new age of the formation of the Isthmus at 2.8 million years ago (Ma). Here, we reject both of these conclusions.
Project description:In a recent paper (Couzi <i>et al.</i> 2018 <i>R. Soc. open sci.</i> <b>5</b>, 180058. (doi:10.1098/rsos.180058)), we proposed a new phenomenological model to account for the I?II?"III" phase sequence in incommensurate <i>n</i>-alkane/urea inclusion compounds, which represents an alternative interpretation to that proposed in work of Toudic <i>et al.</i> In a Comment (Toudic <i>et al.</i> 2019 <i>R. Soc. open sci.</i> <b>6</b>, 182073. (doi:10.1098/rsos.182073)), Toudic <i>et al.</i> have questioned our assignment of the superspace group of phase II of <i>n</i>-nonadecane/urea, which they have previously assigned, based on a (3 + 2)-dimensional superspace, as C222<sub>1</sub>(00<i>?</i>)(10<i>?</i>). In this Reply, we present new results from a comprehensive synchrotron single-crystal X-ray diffraction study of <i>n</i>-nonadecane/urea, involving measurements as a detailed function of temperature across the I?II?"III" phase transition sequence. Our results demonstrate conclusively that "main reflections" (<i>h, k, l</i>, 0) with <i>h+k</i> odd are observed in phase II of <i>n</i>-nonadecane/urea (including temperatures in phase II that are just below the transition from phase I to phase II), in full support of our assignment of the (3+1)-dimensional superspace group P2<sub>1</sub>2<sub>1</sub>2<sub>1</sub>(00<i>?</i>) to phase II. As our phenomenological model is based on phase II and phase "III" of this incommensurate material having the <i>same</i> (3+1)-dimensional superspace group P2<sub>1</sub>2<sub>1</sub>2<sub>1</sub>(00<i>?</i>), it follows that the new X-ray diffraction results are in full support of our phenomenological model.