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Sustained glycolytic oscillations in individual isolated yeast cells - III

ABSTRACT: Sustained glycolytic oscillations in individual isolated yeast cells - III

SUBMITTER: Franco du Preez

PROVIDER: 82 | FAIRDOMHub | 2012-07-23

REPOSITORIES: FAIRDOMHub

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Sustained glycolytic oscillations in individual isolated yeast cells.

Gustavsson Anna-Karin AK van Niekerk David D DD Adiels Caroline B CB du Preez Franco B FB Goksör Mattias M Snoep Jacky L JL

The FEBS journal 20120611 16

<h4>Unlabelled</h4>Yeast glycolytic oscillations have been studied since the 1950s in cell-free extracts and intact cells. For intact cells, sustained oscillations have so far only been observed at the population level, i.e. for synchronized cultures at high biomass concentrations. Using optical tweezers to position yeast cells in a microfluidic chamber, we were able to observe sustained oscillations in individual isolated cells. Using a detailed kinetic model for the cellular reactions, we simu ...[more]

PMID: 22607453

Publication: 1/3

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Project description:Wolf2000 - Cellular interaction on glycolytic oscillations in yeast A two-cell model of glycolysis. This model is described in the article: Effect of cellular interaction on glycolytic oscillations in yeast: a theoretical investigation. Wolf J, Heinrich R. Biochem. J. 2000 Jan; 345 Pt 2: 321-334 Abstract: On the basis of a detailed model of yeast glycolysis, the effect of intercellular dynamics is analysed theoretically. The model includes the main steps of anaerobic glycolysis, and the production of ethanol and glycerol. Transmembrane diffusion of acetaldehyde is included, since it has been hypothesized that this substance mediates the interaction. Depending on the kinetic parameter, the single-cell model shows both stationary and oscillatory behaviour. This agrees with experimental data with respect to metabolite concentrations and phase shifts. The inclusion of intercellular coupling leads to a variety of dynamical modes, such as synchronous oscillations, and different kinds of asynchronous behavior. These oscillations can co-exist, leading to bi- and tri-rhythmicity. The corresponding parameter regions have been identified by a bifurcation analysis. The oscillatory dynamics of synchronized cell populations are investigated by calculating the phase responses to acetaldehyde pulses. Simulations are performed with respect to the synchronization of two subpopulations that are oscillating out of phase before mixing. The effect of the various process on synchronization is characterized quantitatively. While continuous exchange of acetaldehyde might synchronize the oscillations for appropriate sets of parameter values, the calculated synchronization time is longer than that observed experimentally. It is concluded either that addition to the transmembrane exchange of acetaldehyde, other processes may contribute to intercellular coupling, or that intracellular regulator feedback plays a role in the acceleration of the synchronization. for appropriate sets of parameter values, the calculated synchronization time is longer than that observed experimentally. It is concluded either that addition to the transmembrane exchange of acetaldehyde, other processes may contribute to intercellular coupling, or that intracellular regulator feedback plays a role in the acceleration of the synchronization. This model is hosted on BioModels Database and identified by: BIOMD0000000691. To cite BioModels Database, please use: Chelliah V et al. BioModels: ten-year anniversary. Nucl. Acids Res. 2015, 43(Database issue):D542-8. To the extent possible under law, all copyright and related or neighbouring rights to this encoded model have been dedicated to the public domain worldwide. Please refer to CC0 Public Domain Dedication for more information.

Dataset Information

Sustained glycolytic oscillations in individual isolated yeast cells - III

Publications

Sustained glycolytic oscillations in individual isolated yeast cells.

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