Genomics

Dataset Information

161

Human TFIIH kinase CDK7 regulates transcription-associated epigenetic modification


ABSTRACT: CDK7 phosphorylates the RNA polymerase II (pol II) CTD and activates the P-TEFb- associated kinase, CDK9, but its regulatory roles remain obscure. Using human CDK7 analog-sensitive (CDK7as) cells, we observed reduced capping enzyme recruitment, increased pol II promoter-proximal pausing, and defective termination at gene 3'-ends upon CDK7 inhibition. We also found that CDK7 regulates chromatin modifications downstream of transcription start sites. H3K4me3 spreading was restricted at gene 5'-ends and H3K36me3 was displaced toward gene 3'-ends in CDK7as cells. Together, these results implicate a CDK7-dependent "CTD code" that regulates epigenetic marks in addition to RNA processing and pol II pausing. Overall design: WT and analogue sensitive Cdk7as mutant cells were treated with the ATP analogue NM-PP1 that specifically inhibits the Cdk7as mutant kinase. Using ChIP-seq and RNA-seq we tested the effects of Cdk7 inactivation on pol II distribution along genes, CTD Ser2 and Ser5 phosphorylation, capping enzyme recruitment, histone H3K4 and H3K36 methylation and mRNA expression

INSTRUMENT(S): Illumina HiSeq 4000 (Homo sapiens)

SUBMITTER: David L. Bentley  

PROVIDER: GSE100040 | GEO | 2017-08-01

SECONDARY ACCESSION(S): PRJNA390543

REPOSITORIES: GEO

altmetric image

Publications


CDK7 phosphorylates the RNA polymerase II (pol II) C-terminal domain CTD and activates the P-TEFb-associated kinase CDK9, but its regulatory roles remain obscure. Here, using human CDK7 analog-sensitive (CDK7as) cells, we observed reduced capping enzyme recruitment, increased pol II promoter-proximal pausing, and defective termination at gene 3' ends upon CDK7 inhibition. We also noted that CDK7 regulates chromatin modifications downstream of transcription start sites. H3K4me3 spreading was rest  ...[more]

Similar Datasets

2015-12-11 | E-GEOD-62171 | ArrayExpress
2013-12-16 | E-MTAB-1528 | ArrayExpress
2015-04-02 | E-GEOD-67483 | ArrayExpress
| GSE75301 | GEO
2015-04-23 | E-GEOD-60358 | ArrayExpress
2010-05-20 | E-GEOD-21917 | ArrayExpress
2010-05-20 | GSE21917 | GEO
| GSE97827 | GEO
| GSE83412 | GEO
2012-02-19 | GSE27425 | GEO