Genomics

Dataset Information

0

Classifying Drosophila Olfactory Projection Neuron Subtypes by Single-cell RNA Sequencing


ABSTRACT: How a neuronal cell type is defined and how this relates to its transcriptome are still open questions. The Drosophila olfactory projection neurons (PNs) are among the bestcharacterized neuronal types: Different PN classes target dendrites to distinct olfactory glomeruli and PNs of the same class exhibit indistinguishable anatomical and physiological properties. Using single-cell RNA-sequencing, we comprehensively characterized the transcriptomes of 40 PN classes and unequivocally identified transcriptomes for 6 classes. We found a new lineage-specific transcription factor that instructs PN dendrite targeting. Transcriptomes of closely-related PN classes exhibit the largest difference during circuit assembly, but become indistinguishable in adults, suggesting that neuronal subtype diversity peaks during development. Genes encoding transcription factors and cell-surface molecules are the most differentially expressed, indicating their central roles in specifying neuronal identity. Finally, we show that PNs use highly redundant combinatorial molecular codes to distinguish subtypes, enabling robust specification of cell identity and circuit assembly.

ORGANISM(S): Drosophila melanogaster

PROVIDER: GSE100058 | GEO | 2017/10/21

SECONDARY ACCESSION(S): PRJNA390607

REPOSITORIES: GEO

Similar Datasets

2020-11-11 | GSE161228 | GEO
2019-12-19 | GSE140093 | GEO
2019-11-06 | MSV000084543 | MassIVE
2022-04-21 | GSE200841 | GEO
2019-03-06 | GSE127724 | GEO
2020-12-25 | GSE156180 | GEO
2023-11-05 | GSE246726 | GEO
2018-12-06 | GSE107586 | GEO
2022-04-01 | MSV000089187 | MassIVE
| E-GEOD-43617 | biostudies-arrayexpress