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UPF1 helicase promotes Tudor-SN-mediated miRNA decay

ABSTRACT: The helicase activity of UPF1 remodels miRNPs so as to render miRNAs susceptible to Tudor-SN (TSN)-mediated miRNA decay

ORGANISM(S): Homo sapiens

PROVIDER: GSE100068 | GEO | 2017/08/22

SECONDARY ACCESSION(S): PRJNA390626

REPOSITORIES: GEO

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Gene expression in Tudor-SN knockdown Drosophila in normoxic and hypoxic conditions

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Transcriptome changes caused by Tudor-SN mutations in rice seed

Project description:One OsTudor-SN mutant, EM1084, carrying E503K point mutation and one associated WT-OsTSN complemented transgenic (pHL8042/EM1084 ) were applied in this study. EM1084 was obtained by TILLING studies (http://www.shigen.nig.ac.jp/rice/oryzabase). The E503K mutation site in EM1084 lies within the SN3 domain, while the EM1084 complemented transgenic plant was complemented with the full-length WT OsTSN gene driven by native OsTSN promoter. This mutation results in affecting storage protein mRNA localization and, in turn, with a strong reduction in grain weight and storage protein accumulation. Transcriptome analysis on the dehulled 10-14 days old developing seeds from wild type, EM1084 and pHL8042/EM1084 indicates that partial loss of Tudor-SN function caused the differential expression of storage protein genes and relevant genes involved in several essential biological processes during rice development.

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The RNA helicase UPF1 associates with mRNAs cotranscriptionally

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Project description:Gene expression in Tudor-SN knockdown Drosophila in normoxic and hypoxic conditions

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Project description:UPF1 helicase promotes Tudor-SN-mediated miRNA decay

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