Immuno-Detection by sequencing (ID-seq) enables large-scale high- dimensional phenotyping in cells

ABSTRACT: We developed a sequencing-based immunodetection method which makes it possible to multiplex measurements of many different epitopes from proteins and phosphoproteins in a high throughput setting. With these DNA-tagged antibodies and dedicated sample preparation protocol, we studied the effect of hundreds of kinase inhibitors on 70 (phospho-)proteins in primary human skin cells.

ORGANISM(S): synthetic construct Homo sapiens

PROVIDER: GSE100135 | GEO | 2017/07/01

SECONDARY ACCESSION(S): PRJNA390781

REPOSITORIES: GEO