Genomics

Dataset Information

37

Pathways change in expression during replicative aging in Saccharomyces cerevisiae


ABSTRACT: Yeast replicative aging is a process resembling replicative aging in mammalian cells. During aging, wild type haploid yeast cells enlarge, become sterile, and undergo nucleolar enlargement and fragmentation; we sought gene expression changes during the time of these phenotypic changes. Gene expression studied via microarrays and qPCR has shown reproducible, statistically significant changes in mRNA of genes at 12 and 18-20 generations. Our findings support previously described changes towards aerobic metabolism, decreased ribosome gene expression, and a partial Environmental Stress Response. Our novel findings include a pseudo-stationary phase, down-regulation of methylation-related metabolism, increased Nucleotide Excision Repair related mRNA, and a strong up-regulation of many of the regulatory subunits of protein phosphatase I (Glc7). These findings are correlated with aging changes in higher organisms as well as with the known involvement of protein phosphorylation states during yeast aging. J Gerontol, Jan, 2008, vol 63A, no. 1. Keywords: aging time course Overall design: Comparison of gene expression in yeast aging in a mating type W303 wild type using seven microarrays each of 1, 12, and 18-20 generations and six microarrays of 8 generations. In each case, conditions-matched control young cell samples were used to avoid differential induction of stress response genes.

INSTRUMENT(S): WashU Operon Array-Ready Yeast Oligo set (Yeast_V1.1.2)

SUBMITTER: Laura L. Mays Hoopes  

PROVIDER: GSE10018 | GEO | 2008-02-01

SECONDARY ACCESSION(S): PRJNA108207

REPOSITORIES: GEO

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Pathways change in expression during replicative aging in Saccharomyces cerevisiae.

Yiu Gloria G   McCord Alejandra A   Wise Alison A   Jindal Rishi R   Hardee Jennifer J   Kuo Allen A   Shimogawa Michelle Yuen MY   Cahoon Laty L   Wu Michelle M   Kloke John J   Hardin Johanna J   Mays Hoopes Laura L LL  

The journals of gerontology. Series A, Biological sciences and medical sciences 20080101 1


Yeast replicative aging is a process resembling replicative aging in mammalian cells. During aging, wild-type haploid yeast cells enlarge, become sterile, and undergo nucleolar enlargement and fragmentation; we sought gene expression changes during the time of these phenotypic changes. Gene expression studied via microarrays and quantitative real-time reverse-transcription polymerase chain reaction (qPCR) has shown reproducible, statistically significant changes in messenger RNA (mRNA) of genes  ...[more]

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