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A COUP-TFII human embryonic stem cell reporter line to identify and purify atrial cardiomyocytes

ABSTRACT: Here, we targeted mCherry to the COUP-TFII genomic locus in NKX2.5eGFP/+ hPSCs. Upon differentiation to atrial and ventricular cardiomyocytes (CMs) this dual atrial COUP-TFIImCherry/+-NKX2.5eGFP/+ reporter line allowed identification and selection of GFP+ (G+)/mCherry+ (M+) CMs following cardiac differentiation. These cells exhibited transcriptional and functional properties of atrial CMs, whereas G+/M- CMs displayed ventricular characteristics. Via CRISPR/Cas9-mediated knockout, we demonstrated that COUP-TFII is not required atrial specification in hPSCs.

ORGANISM(S): Homo sapiens

PROVIDER: GSE100199 | GEO | 2017/12/31

SECONDARY ACCESSION(S): PRJNA390975

REPOSITORIES: GEO

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Atrial Identity Is Determined by A COUP-TFII Regulatory Network (ChIP-Seq)

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