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Next-generation sequencing measuring genome-wide RNA decay by s4U Seq in naïve CD4+ T cells from Mettl3 WT and KO mice

ABSTRACT: Naïve T cells of Mettl3 KO and WT were pulse labeled by s4U for 15 min before and after IL-7 stimulation for 15min/30min/45min/60min/75min/90min. The total input RNAs or s4U nascent RNAs were isolated and prepared for next-generation sequencing. The RNA response to IL-7 and RNA decay rates were then modeled and calculated, to infer the m6A/Mettl3 effects.

ORGANISM(S): Mus musculus

PROVIDER: GSE100278 | GEO | 2017/08/17

SECONDARY ACCESSION(S): PRJNA391351

REPOSITORIES: GEO

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