Dataset Information


Therapeutic antibodies to ganglioside GD2 evolved from highly selective germline antibodies VI

ABSTRACT: Purpose: This study uses a high-throughput glycan microarray to evaluate the immunological evolution of antibodies to the glyco-antigen GD2. The goal is to determine germline and affinity mature antibody specificity and affinities/ Results: Affinity mature anti-GD2 antibodies 3F8 and ch14.18 had high affinity and were highly specific for the target GD2. Germline antibodies were also hihgly specific and had surprisingly high affinity. Conclusion: Antibodies to GD2 evolved from highly specific germlines. Highly specific germlines may be critical in evading autoimmunity issues. Overall design: Germline variable regions of 3F8 and ch14.18 were determined. Germline and affinity mature antibody DNA were synthesized and transfected into HEK293 cell lines. Secreted antibodies were purified from supernatant using affinity chromatography. Antibodies were evaluated on multiple assay platforms, including glycan microarrays, protein microarrays, and cell binding assays.


SUBMITTER: Jeff Gildersleeve 

PROVIDER: GSE100309 | GEO | 2017-08-25



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Therapeutic Antibodies to Ganglioside GD2 Evolved from Highly Selective Germline Antibodies.

Sterner Eric E   Peach Megan L ML   Nicklaus Marc C MC   Gildersleeve Jeffrey C JC  

Cell reports 20170801 7

Antibodies play a crucial role in host defense and are indispensable research tools, diagnostics, and therapeutics. Antibody generation involves binding of genomically encoded germline antibodies followed by somatic hypermutation and in vivo selection to obtain antibodies with high affinity and selectivity. Understanding this process is critical for developing monoclonal antibodies, designing effective vaccines, and understanding autoantibody formation. Prior studies have found that antibodies t  ...[more]

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