Genomics

Dataset Information

0

Single-Cell Transcriptomics and Fate Mapping of Ependymal Cells Reveals an Absence of Neural Stem Cell Function


ABSTRACT: Ependymal cells are multi-ciliated cells that form the brain’s ventricular epithelium and a niche for neural stem cells (NSCs) in the ventricular-subventricular zone (V-SVZ). In addition, ependymal cells are suggested to be latent NSCs with a capacity to acquire neurogenic function. This remains highly controversial due to a lack of prospective in vivo labeling techniques that can effectively distinguish ependymal cells from neighboring V-SVZ NSCs. We describe a transgenic system that allows for targeted labeling of ependymal cells within the V-SVZ. Single-cell RNA-seq revealed that ependymal cells are enriched for cilia-related genes and share several stem-cell-associated genes with neural stem or progenitors. Under in vivo and in vitro neural-stem- or progenitor-stimulating environments, ependymal cells failed to demonstrate any suggestion of latent neural-stem-cell function. These findings suggest remarkable stability of ependymal cell function and provide fundamental insights into the molecular signature of the V-SVZ niche.

ORGANISM(S): Mus musculus

PROVIDER: GSE100320 | GEO | 2018/05/02

REPOSITORIES: GEO

Similar Datasets

2020-02-19 | GSE137852 | GEO
2021-05-01 | GSE118009 | GEO
2023-10-16 | GSE229552 | GEO
2023-10-16 | GSE229276 | GEO
| E-GEOD-22476 | biostudies-arrayexpress
| E-GEOD-21208 | biostudies-arrayexpress
2011-12-31 | GSE21208 | GEO
2023-10-16 | GSE229549 | GEO
2023-07-13 | GSE214983 | GEO
| E-GEOD-76968 | biostudies-arrayexpress