Transcriptomics,Genomics

Dataset Information

160

Expression profiling of lncRNAs, miRNAs and mRNAs and their differential expression in leiomyoma using next generation RNA sequencing


ABSTRACT: Total RNA was isolated from leiomyoma and paired myometrium (N=8) and samples from three pairs were subjected to RNA sequencing. After analysis, 5941 lncRNAs (2813 up- and 3128 down-regulated at ≥1.5 fold), 148 miRNAs (56 up- and 96 down-regulated at ≥1.5 fold) and 3855 mRNAs (2030 up- and 1855 down-regulated at ≥1.5 fold) were differentially expressed in leiomyomas. Using QRT-PCR we further confirmed the expression of HULC, lnc-MEG3, LINC00890, TSIX, LINC00473, lnc-KLF9-1 and lnc-POTEM-3 (lncRNA-ATB) in leiomyoma and matched myometrium (N=8). Our results presented here provide a comprehensive expression profile of lncRNAs in leiomyomas with concurrent integrated expression of miRNAs and mRNAs in leiomyomas. Overall design: Identify the expression profile of lncRNAs with concurrent assessment of miRNAs and mRNAs profiles in leiomyomas and paired myometrium using next generation RNA sequencing

INSTRUMENT(S): Illumina HiSeq 3000 (Homo sapiens)

SUBMITTER: Tsai-Der Chuang  

PROVIDER: GSE100338 | GEO | 2017-06-22

SECONDARY ACCESSION(S): PRJNA391373

REPOSITORIES: GEO

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Publications

Expression Profiling of lncRNAs, miRNAs, and mRNAs and Their Differential Expression in Leiomyoma Using Next-Generation RNA Sequencing.

Chuang Tsai-Der TD   Khorram Omid O  

Reproductive sciences (Thousand Oaks, Calif.) 20170607 2


The objective of this study was to identify the expression profile of long noncoding RNAs (lncRNAs) with concurrent assessment of microRNA (miRNA) and messenger RNA (mRNA) profiles in leiomyomas and paired myometrium using next-generation RNA sequencing and assembly of RNA transcripts. Total RNA was isolated from leiomyoma and paired myometrium (N = 8) and samples from 3 pairs were subjected to RNA sequencing. Normalized assembly of over 48 000 lncRNAs resulted in identification of 45 936 lncRNA  ...[more]

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