Transcriptomics,Genomics

Dataset Information

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Characterization of small RNAs expressed in roots and galls from Arabidopsis thaliana induced by the plant parasitic nematodes Meloidogyne incognita


ABSTRACT: By sequencing small RNAs from uninfected Arabidopsis roots and from galls seven and 14 days post infection with Meloidogyne incognita, we sequenced by SOLiD technology the RNA fraction below 50nt. We identified 24 miRNAs differentially expressed in gall as putative regulators of gall development. Overall design: Uninfected Arabidopsis roots and galls seven and 14 days post infection with Meloidogyne incognita (3 replicates)

INSTRUMENT(S): AB 5500xl Genetic Analyzer (Arabidopsis thaliana)

SUBMITTER: Kevin Lebrigand  

PROVIDER: GSE100498 | GEO | 2017-10-04

SECONDARY ACCESSION(S): PRJNA391955

REPOSITORIES: GEO

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Publications

Characterization of microRNAs from Arabidopsis galls highlights a role for miR159 in the plant response to the root-knot nematode Meloidogyne incognita.

Medina Clémence C   da Rocha Martine M   Magliano Marc M   Ratpopoulo Alizée A   Revel Benoît B   Marteu Nathalie N   Magnone Virginie V   Lebrigand Kevin K   Cabrera Javier J   Barcala Marta M   Silva Ana Cláudia AC   Millar Anthony A   Escobar Carolina C   Abad Pierre P   Favery Bruno B   Jaubert-Possamai Stéphanie S  

The New phytologist 20170914 3


Root knot nematodes (RKN) are root parasites that induce the genetic reprogramming of vascular cells into giant feeding cells and the development of root galls. MicroRNAs (miRNAs) regulate gene expression during development and plant responses to various stresses. Disruption of post-transcriptional gene silencing in Arabidopsis ago1 or ago2 mutants decrease the infection rate of RKN suggesting a role for this mechanism in the plant-nematode interaction. By sequencing small RNAs from uninfected A  ...[more]

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