Transcriptomics

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Comparative cardiac induction time-courses using wild-type or genetically modified human embryonic stem cells


ABSTRACT: This study compares directed cardiac differentiation time-courses using (i) HuES6 cells with endogenous ISL1 knockout + inducible ISL1 transgene, and (ii) wild-type HuES6 cells. For the first series, a subset of samples was left untreated (= ISL1 knockout scenario) while another subset received doxycycline treatment to upregulate ISL1 at days 3 and 4 of differentiation (ISL1 rescue). For the second series, a subset of samples was differentiated under default conditions (no RA) while another subset received retinoic acid (RA) on days 3 and 4 of differentiation, which leads to an atrial-like phenotype.

ORGANISM(S): Homo sapiens

PROVIDER: GSE100592 | GEO | 2018/01/13

SECONDARY ACCESSION(S): PRJNA392243

REPOSITORIES: GEO

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