Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Large-scale tethering of RNA binding proteins discovers alternative splicing regulators

ABSTRACT: This SuperSeries is composed of the SubSeries listed below.

ORGANISM(S): Homo sapiens

PROVIDER: GSE100638 | GEO | 2025/04/27

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

Large-scale tethering of RNA binding proteins discovers alternative splicing regulators [microarray]

Project description:To elucidate the role of large sets of RBPs, we developed a comprehensive and diverse plasmid resource3 of 765 RBPs fused to the MS2 bacteriophage coat protein. When systematically tethered to an intronic 4 MS2 hairpin downstream of an alternative exon, we identified 55 RBPs that significantly induced exon 5 retention.

2025-04-27 | GSE100600 | GEO

Large-scale tethering of RNA binding proteins discovers alternative splicing regulators [RIP-Seq]

Project description:To elucidate the role of large sets of RBPs, we developed a comprehensive and diverse plasmid resource3 of 765 RBPs fused to the MS2 bacteriophage coat protein. When systematically tethered to an intronic 4 MS2 hairpin downstream ofan alternative exon, we identified 55 RBPs that significantly induced exon 5 retention.

2025-04-27 | GSE100637 | GEO

Large-scale tethering of RNA binding proteins discovers alternative splicing regulators

Project description:Large-scale tethering of RNA binding proteins discovers alternative splicing regulators

| PRJNA392334 | ENA

Large-scale RNA-binding protein tethering screen discovers modulators of circular RNA metabolism

Project description:This SuperSeries is composed of the SubSeries listed below.

2026-08-28 | GSE314435 | GEO

Large-scale RNA-binding protein tethering screen discovers modulators of circular RNA metabolism [screen_BY]

Project description:Circular RNAs (circRNAs), formed by back-splicing in mammalian cells, are increasingly recognized for their roles in various biological processes and their potential in RNA therapeutics. However, the RNA-binding proteins (RBPs) that regulate circRNAs throughout their lifecycle remain largely unknown. Here, we present the most comprehensive evaluation to date, assessing 730 RBPs for their potential to regulate circRNAs. Using both luciferase-based and qPCR assays, we uncover a range of potential regulatory mechanisms. We identify 296 RBPs that encompass diverse components of circRNA metabolism including biogenesis, nuclear export, translation and degradation. Our findings show that LARP4B universally enhances circRNA translation, while ANXA2 enhances circRNA translation in a cell type-specific manner, shedding light on advancing circRNA therapeutic vector design. Moreover, we identify RBM15 as a multifaceted regulator of circRNAs, promoting their biogenesis via interactions with SRSF proteins and retaining circRNAs in the nucleus through RNA-dependent interactions with IGF2BP1. Notably, RBM15 binds directly to exonic regions near back-splicing junctions, and is a conserved RBP regulator of circRNA biogenesis. These insights advance our understanding of the trans-regulators in circRNA biogenesis and metabolism and offer new avenues for RNA therapeutic development.

2026-08-28 | GSE277896 | GEO

Large-scale RNA-binding protein tethering screen discovers modulators of circular RNA metabolism [tethering_screen_fly_total_RNA_seqs]

2026-08-28 | GSE314434 | GEO

Large-scale RNA-binding protein tethering screen discovers modulators of circular RNA metabolism [tethering_screen_RBM15_OE_KD_total_RNA_seq]

2026-08-28 | GSE314433 | GEO