Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

RNA-seq of chicken small yellow follicles with different FSHR expression level

ABSTRACT: The aim of study was to identify the differentially expressed genes in SYF by using Illumina RNA deep sequencing, one SYF has the highest expression level of FSHR, the other has the lowest expression level of FSHR. We sampled six follicles from three chickens.In order to discover the key genes that regulate the process of follicle selection. From RNA-seq results, we got 26.63G reads,when mapping the reads to chicken genome, 75.13%-78.40% reads were successfully mapped, and more than 98% reads were unique.and we found 1382 new genes,functional annotation of the new gene was also performed.

ORGANISM(S): Gallus gallus

PROVIDER: GSE100673 | GEO | 2017/12/06

SECONDARY ACCESSION(S): PRJNA392469

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Similar Datasets

C. elegans gene expression in healthy and PA14-infected wild-type and fshr-1 mutant worms

Project description:Analysis of gene expression in worms exposed to PA14 for 4 hours. Worms used were wild-type or fshr-1(ok778) mutants. Comparisons allowed determination of fshr-1-dependent gene expression.

2015-09-30 | GSE72029 | GEO

Chicken small yellow and F6 follicles LC-MSMS

Project description:we compared the phosphoproteomes of SY and F6 follicles in laying hens and identified several genes involved in chicken follicle selection.

2022-09-01 | PXD029503 | Pride

DENND1A Desensitizes Granulosa Cells to FSH by Arresting Intracellular FSHR Transportation

Project description:Genome-wide association studies (GWAS) have identified polycystic ovary syndrome (PCOS)-associated loci, including DENND1A encoding a clathrin-binding protein involved in vesicle transport as a guanine nucleotide exchange factor. The effect of DENND1A on reproduction and whether it participates in follicle development disorder and androgen synthesis in PCOS remain unknown. Here, we demonstrated that DENND1A expression increased in ovarian granulosa cells (GCs) of PCOS patients and was positively correlated with serum testosterone concentration. We constructed a systemic transgenic Dennd1a mouse (TG mouse) model, which showed subfertility, irregular estrous cycles, and increased production of testosterone after PMSG stimulation. Moreover, TG mice showed hyporesponsiveness to PMSG with smaller ovary size and less well-developed follicles. Intracellular follicle-stimulating hormone receptor (FSHR) transport was disturbed by overexpression of Dennd1a, which promoted FSHR internalization and inhibited its recycling. Our findings revealed the reproductive function of DENND1A and the underlying mechanisms, which provided new insights into the PCOS pathogenesis and contributing to drug design for PCOS treatment.

2023-07-05 | GSE236248 | GEO

Modeling FSHR function with hPSCs

Project description:Follicle-stimulating hormone (FSH) is crucial in the development and regulation of reproductive functions. We expressed MAC-tagged FSH receptor (FSHR) in HEK293 cells followed by affinity purification mass spectrometry analyses, to investigate interactome of FSHR at resting state and upon FSH stimulation. In total, 19 specific high-confidence interactors for WT FSHR and 14 for A189V FSHR, several of which have been linked to infertility.

2021-12-17 | MSV000088577 | MassIVE

Transcriptome Analysis on Single Small Yellow Follicles Reveals That <i>Wnt4</i> Is Involved in Chicken Follicle Selection.

Project description: Not available

| S-EPMC5694752 | biostudies-literature

The Common Follicle-Stimulating Hormone Receptor (FSHR) Promoter Polymorphism FSHR -29G?>?A Affects Androgen Production in Normal Human Small Antral Follicles.

Project description: Not available

| S-EPMC5454067 | biostudies-literature

Integrated transcriptomic analysis on small yellow follicles reveals that sosondowah ankyrin repeat domain family member A inhibits chicken follicle selection.

Project description: Not available

| S-EPMC8255886 | biostudies-literature

Differential genome-wide gene expression profiling of bovine largest and second largest follicles

Project description:We tried to classify largest (F1) and second largest follicles (F2) by global gene expression profiles and to identify differentially expressed genes between the follicular groups using combination of microarray and quantitative real-time PCR (QPCR) analysis. F1 (10.7 ± 0.7 mm) and F2 (7.8 ± 0.2 mm) were collected for microarray and QPCR analysis. Global gene expression profiles of each follicle were analyzed by hierarchical cluster analysis and expression profiles of representative 16 genes were confirmed by QPCR analysis. Hierarchical cluster analysis classified the follicles into two clusters by microarray expression profiles. The cluster A was composed of only F2 and was characterized by highly expression of 31 genes including IGFBP5, whereas the cluster B contained only F1 and was predominately expressed 45 genes including CYP19, FSHR and INHA. The QPCR analysis confirmed AMH, CYP19, FSHR, GPX3, PGF, PLA2G1B, SCD and TRB2 were greater in F1 than F2, while CCL2, GADD45A, IGFBP5, PLAUR, SELP, SPP1, TIMP1 and TSP2 were greater in F2 than F1. Expression of stage-specific genes in follicles may be closely associated with its growth or atresia. Several genes identified in this study will provide intriguing candidates for the determinant of follicular growth. Global gene expression of three largest follicles and two second largest follicles were analyzed

2010-03-31 | E-GEOD-18145 | biostudies-arrayexpress

MicroRNAs expressed from FSHR and aromatase genes target important ovarian functions.

Project description:MicroRNAs expressed from FSHR and aromatase genes target important ovarian functions.

| PRJNA506226 | ENA

FSH and eCG impact follicles development and expression of ovarian FSHR and caspase-9 in mice.

Project description: Not available

| S-EPMC5534248 | biostudies-literature