Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

RNA-Seq of different Clytia female gonad tissues and oocytes at different growth stage

ABSTRACT: We aimed to quantify in silico the expression level of candidate genes in different Clytia gonad tissues and oocytes at different growth stage

ORGANISM(S): Clytia hemisphaerica

PROVIDER: GSE101072 | GEO | 2018/01/03

SECONDARY ACCESSION(S): PRJNA393679

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Similar Datasets

RNA-seq from oocytes and zygotes of pabpn1l ko and wt female mice during different stages

Project description:PABPN1L participates in the process of post-transcriptional regulation and degradation of maternal mRNA.So,we collect samples of each period from pabpn1l ko and wt female mice during different stages.Then, RNA was extracted and detected.Reverse transcription was performed on the samples that met the effective concentration.After quality inspection, build the library.GV oocytes, MII oocytes and zygotes were collected from three different PABPN1L KO or WT mice separately.

2021-03-19 | E-MTAB-10199 | biostudies-arrayexpress

RNA sequencing of small number germ cells including primordial germ cells (PGCs) collected from female embryonic 12.5 day (E12.5), Female germline stem cells (FGSCs), Germinal-stage oocytes (GV oocytes) and Meiosis II oocytes (MII oocytes)

Project description:Mammalian oogenesis is an intricate and coordinated process involving dramatic changes in the transcriptional architecture. Through the small number of cells RNA sequencing (RNA Seq), we comprehensively uncovered the transcriptome dynamics of the mouse PGCs, FGSCs, GV Oocytes and M II Oocytes using. These results would provide some valuable navigation for the mechanism research of mammalian FGSCs in the future.

2017-08-15 | GSE75738 | GEO

RNA-seq of oocytes and zygotes from pabpn1l ko and wt female mice

Project description:PABPN1L participates in the process of post-transcriptional regulation and degradation of maternal mRNA.We collect samples of each period from pabpn1l ko and wt female mice during different stages. Each group contained a total of 30 oocytes or zygotes. In the initial step, we spiked 2 × 106 mRNA-EGFP, transcribed in vitro, into each group.Then, RNA was extracted and detected.Reverse transcription was performed on the samples that met the effective concentration.After quality inspection, build the library.GV oocytes, MII oocytes and zygotes were collected from three different PABPN1L KO or WT mice seperately.

2021-03-26 | E-MTAB-10233 | biostudies-arrayexpress

Transcriptome profiles of porcine oocytes with different developmental competence

Project description:the objectives of this study was to identify and analyze the transcriptome profiles of porcine oocytes aspirated from follicles of different sizes which showed differences in developmental competence and chromatin configurations using RNA high throughput sequencing technology

2019-11-25 | GSE129994 | GEO

Transcriptome profiles of porcine oocytes with different developmental competence

Project description:Transcriptome profiles of porcine oocytes with different developmental competence

| PRJNA533463 | ENA

Transcription profiles of cumulus cells from GnRH agonists and GnRH antagonists treated oocytes at different maturity stages

Project description:Affymetrix gene expression profiling in cumulus cells (CC) retrieved from patients undergoing GnRH agonists and GnRH antagonists IVF treatment. Oocytes from three different maturity stages were considered: metaphase I oocytes (MI), nonfertilized metaphase II (MII) oocytes (MII-NF) and MII oocytes developed to blactocyst stage embryo (MII-BL).

2013-09-13 | GSE34230 | GEO

Female mating status influences protein composition of mature Drosophila oocytes

Project description:Oocyte composition can directly influence offspring fitness, particularly in oviparous species such as most insects, where it is the primary form of parental investment. Oocyte production is also energetically costly, dependent on female condition and responsive to external cues. We investigated whether mating influences mature oocyte composition in Drosophila melanogaster using a quantitative proteomic approach. Our analyses robustly identified 4,485 oocyte proteins and revealed that stage-14 oocytes from mated females differed significantly in protein composition relative to oocytes from unmated females. Proteins forming a highly interconnected network enriched for translational machinery and transmembrane proteins were increased in oocytes from mated females, including calcium binding and transport proteins. This mating-induced modulation of oocyte maturation was also significantly associated with proteome changes that are known to be triggered by egg activation. We propose that these compositional changes are likely to have fitness consequences and adaptive implications given the importance of oocyte composition, rather than active gene expression, to the maternal-to-zygotic transition and early embryogenesis.

2021-02-24 | PXD022142 | Pride

Expression data from different stages and types of oocytes and cumulus cells (CCs)

Project description:The bidirectional communication between bovine oocytes and CCs is vital for functioning and development of both cell types. We used microarray to identify genes which are differentially expressed between germinal vesicle (GV)- and metaphase II (MII)-stage oocytes and CCs and those differentially expressed when oocytes mature with or without the other. We also identified genes differentially expressed between CCs at GV and MII stages. Slaughterhouse ovaries were collected and GV-stage cumulus oocyte complexes (COCs) were aspirated. Different stages and types of oocytes and CCs were used for total RNA isolation and hybridisation on Affymetrix microarray.

2010-03-23 | E-GEOD-21005 | biostudies-arrayexpress

Transcription profiling by array of Drosophila stage 14 oocytes

Project description:Mature stage 14 oocytes prior to egg activation were isolated from wild type Oregon Red fly cultures by a combined blender/ sieving method which allows specific and efficient enrichment of stage 14 oocytes. Total RNA was extracted from 4 replicate collections and submitted to Affymetrix microarray hybridisation.

2011-06-02 | E-MEXP-2746 | biostudies-arrayexpress

RNA-seq of pabpn1l ko and wt female mice oocytes and zygotes

Project description:After the library was qualified, the library was pooled according to the effective concentration and the demand of target offline data, and was sequenced by Illumina platform. Since PABPN1L can bind the poly(A) tail, we propose that it participates in the process of post-transcriptional regulation and degradation of maternal mRNA. We investigated the mRNA changes in GV oocytes, MII oocytes, and fertilized eggs using RNA-seq. GV oocytes, MII oocytes and zygotes were collected and mixed from three mice of each genotypes separately.

2022-08-01 | E-MTAB-9488 | biostudies-arrayexpress

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data