Ionizing radiation induced changes of miRNA expression in A549 lung cancer cells

ABSTRACT: We have employed microarray expression profiling as a discovery platform to identify microRNAs induced by radiation. Human A549 lung cancer cells were irradiated (2Gy/day for consistent 3days) and miRNA signature was identified that distinguished between control and radiation treated samples.Irradiated and un-irradiated A549 cells were harvested after 48 hr of treatment and microarray analysis was performed. Expression of five miRNAs (miR-30a, miR-30b, miR-30c, miR-30d, and miR-30d) from this signature was quantified in the same RNA samples by real-time PCR, confirming variability between control and radiation treated A549 cells.

ORGANISM(S): Homo sapiens

PROVIDER: GSE101085 | GEO | 2017/07/11

SECONDARY ACCESSION(S): PRJNA393691

REPOSITORIES: GEO