Transcriptomics,Genomics

Dataset Information

39

Progerin-induced changes in gene expression


ABSTRACT: The premature aging disease Hutchinson-Gilford Progeria Syndrome (HGPS) is caused by constitutive production of progerin, a mutant form of the nuclear architectural protein lamin A1. Progerin is also sporadically expressed in wild type cells and has been linked to physiological aging. HGPS cells exhibit extensive nuclear defects including abnormal chromatin structure and increased DNA damage. At the organismal level, HGPS affects several tissues particularly of mesenchymal origin. How the cellular defects of HGPS cells lead to the organismal defects has been unclear. To begin to unravel how progerin leads to disease phenotypes, we analyzed time-dependent changes in transcriptional profiles in response to progerin expression in a hTERT-immortalized skin fibroblast cell line expressing either GFP-progerin or the GFP-wt lamin A control Keywords: time course, cell line comparison Overall design: Three time points (0d, 5d, 10d after induction of the exogenous protein) were analyzed for each cell line (expressing either GFP-progerin or GFP-wt lamin A). Two biological replicates were analyzed for each time point.

INSTRUMENT(S): GE Healthcare/Amersham Biosciences CodeLink Human Whole Genome Bioarray

SUBMITTER: paola scaffidi  

PROVIDER: GSE10123 | GEO | 2008-02-09

SECONDARY ACCESSION(S): PRJNA108667

REPOSITORIES: GEO

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Publications

Lamin A-dependent misregulation of adult stem cells associated with accelerated ageing.

Scaffidi Paola P   Misteli Tom T  

Nature cell biology 20080302 4


The premature-ageing disease Hutchinson-Gilford Progeria Syndrome (HGPS) is caused by constitutive production of progerin, a mutant form of the nuclear architectural protein lamin A. Progerin is also expressed sporadically in wild-type cells and has been linked to physiological ageing. Cells from HGPS patients exhibit extensive nuclear defects, including abnormal chromatin structure and increased DNA damage. At the organismal level, HGPS affects several tissues, particularly those of mesenchymal  ...[more]

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