Dataset Information


Aristolochic acid nephropathy induced by aristolochic acid type I in C57BL/6 mice

ABSTRACT: Aristolochic acid (AA) is a nephrotoxic carcinogen responsible for acute kidney injury, chronic renal failure, and associated urothelial cancers. This study aims to determine the genes in xenobiotic metabolism pathway regulated by AA and clarify the molecular mechanism underlying their action. Overall design: Six-week-old female and male C57BL/6 mice were used. AAI (Sigma-Aldrich, St. Louis, MO, USA) was dissolved by corn oil and administered by i.p. injection at a dose of 2 mg/kg/day (male) or 5 mg/kg/day (female), 5 days per week for 3 weeks. Total liver RNA was isolated from four groups of animals, AAI- or corn oil-treated female and male WT mice, using RNeasy Mini Kits (Qiagen Inc., Valencia, CA, USA). In each group, pooled RNA was prepared by mixing the same amount total RNA from 5 mice. The microarray hybridization was performed by Welgen Biotech, Co., Ltd. (Taipei, Taiwan) using Agilent SurePrint Microarray (Agilent Technologies, USA). After microarrays were scanned with an Agilent microarray scanner (Agilent Technologies, USA) for fluorescent signal intensities, scanned images were analyzed by Feature extraction10.5.1.1 software (Agilent Technologies, USA). Raw signal data was normalized by quantile normalization for differentially expressed gene discovery.

INSTRUMENT(S): Agilent-074809 SurePrint G3 Mouse GE v2 8x60K Microarray [Probe Name version]

SUBMITTER: Yi-Ming Arthur Chen 

PROVIDER: GSE101530 | GEO | 2018-05-09


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Glycine N-methyltransferase inhibits aristolochic acid nephropathy by increasing CYP3A44 and decreasing NQO1 expression in female mouse hepatocytes.

Chang Ming-Min MM   Lin Chang-Ni CN   Fang Cheng-Chieh CC   Chen Marcelo M   Liang Peir-In PI   Li Wei-Ming WM   Yeh Bi-Wen BW   Cheng Hung-Chi HC   Huang Bu-Miin BM   Wu Wen-Jeng WJ   Chen Yi-Ming Arthur YA  

Scientific reports 20180503 1

Plants containing aristolochic acids (AA) are nephrotoxins. Glycine N-methyltransferase (GNMT) acts to bind environmental toxins such as benzo(a)pyrene and aflatoxin B1, translocate into nucleus, and alter hepatic metabolism. This study aims to determine the role of GNMT in AA-induced nephropathy. We established an AA nephropathy mouse model and found that AA type I (AAI)-induced nephropathy at a lower concentration in male than in female mice, implying sex differences in AAI resistance. Microar  ...[more]

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