Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Transcriptome of U251 cells knocking down RPL13

ABSTRACT: RPL13 was identified to be downregulated in brain of Alzheimer's disease (AD). To explore the involved pathways in response to RPL13 reduction, we knockdownRPL13 using siRNA in U251 cell line with a stably expressed mutant APP (K670N/M671L, U251-APP cells). RNA-seq (by IlluminaHiseq 4000) and following analyses were then performed to detect genes whose expression were influenced by RPL13 knockdown.

ORGANISM(S): Homo sapiens

PROVIDER: GSE101564 | GEO | 2023/12/31

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Similar Datasets

Identification of downstream genes regulated by YAP1 through knockdown and overexpression of YAP1 in U251 cell with a stably expression of mutant APP

Project description:Upstream regulator genes are central hub nodes in a network and their expression may response to upstream trigger factors of a disease and influence expression of hundreds of downstream genes, and further facilitate disease development. Therefore, the identification of upstream regulator genes are vital for understanding the pathophysiology of the disease and seek for potential therapeutic targets for the treatment of the disease. YAP1 was identified as a candidate upstream regulator for Alzheimer's disease (AD) in our study. To test whether expression alterations of YAP1 can lead to AD expression network disturbance and thus promoting AD progression, we knockdown and overexpressed YAP1 in U251 cell line with a stably expression of mutant APP (K670N/M671L) (U251-APP cells). RNA-seq (by IlluminaHiseq 4000) and following analyses were then performed to detect genes whose expression were influenced by YAP1 expression changes. The results indicated that expression alterations of YAP1 can significantly disturbe the whole AD expression network.

2017-07-06 | GSE100891 | GEO

Transcriptome of U251 cells overexpression complement component 7

Project description:We identified a rare coding variant (p.K420Q) in the complement component 7 (C7) gene affecting the risk of Alzheimer's disease. To investigate the cellular effects of the mutant, we performed RNA-seq in cell line overexpression wilt-type and mutant C7. U251 glioma cells with stable expression of mutant APP (K670N/M671L) (U251-APP cells), which produce Aβ42 under Dox inducing, were used as the model cell. Total RNA of U251-APP cells overexpressing wild type and mutant C7 proteins were subjected to transcriptome sequencing using Illumina Hiseq 4000 platform.

2018-11-22 | GSE101608 | GEO

Expression data from human glioma cells U251 or LN-319 with stable expression of GFP control or sh-Circ-SHPRH

Project description:The cDNA array of U251 or LN-319 cells stably infected with sh-Circ-SHPRH or control lenti-virus.

2017-03-30 | GSE97185 | GEO

Single and double knock-down of ETV6 and NFKB1 in U251 glioblastoma cell line.

Project description:To elucidate the mechanism(s) underlying the synergistic interaction between ETV6 and NFKB1, we analyzed the genome-wide transcriptional consequences of single and double knock-downs of the two TFs in U251 cells.

2016-09-26 | GSE75003 | GEO

U251 LC-MSMS - U251 cells were treated with Vinblastine or Taxol and phosphotyrosine signaling was measured.

Project description:U251 cells were treated with Vinblastine or Taxol and phosphotyrosine signaling was measured.

2018-11-30 | PXD008939 | Pride

Mechanism of Nootkatone in U251

Project description:Nootkatone can induce the changes of gene expression in U251 cells

2023-07-02 | GSE235973 | GEO

Transcriptomic sequencing analysis of glioblastoma cell lines DBTRG and U251.

Project description:We showed that DBTRG is more invasive than U251 cell lines by novel brain-stiffness-mimicking matrix gel invasion platform and transwell invasion assay. To understand the molecular mechanisms of DBTRG being more invasive than U251, we performed transcriptomic sequencing analysis of DBTRG and U251 cell lines.

2022-04-18 | GSE200574 | GEO

Mechanism of Taurochenodeoxycholic acid in U251

Project description:Taurochenodeoxycholic acid can induce the changes of gene expression in U251 cells

2023-07-04 | GSE236200 | GEO

Mechanism of Forsythoside B in U251

Project description:Forsythoside B can induce the changes of gene expression in U251 cells

2023-08-21 | GSE240769 | GEO

Expression data from PARK2 overexpression in U251 cells

Project description:PARK2 (PARKIN) is an E3 ubiquitin ligase whose dysfunction has been associated with the progression of Parkinsonism and human malignancies, and its role in cancer remains to be explored. In this study, we investigated its role in glioma. We used microarrays to detail the global programme of gene expression upon PARK2 overexpression in U251 cells U251 cells with PARK2 overexpression or control (GFP) were subjected for RNA extraction and hybridization on Affymetrix microarrays.

2014-10-03 | E-GEOD-61973 | biostudies-arrayexpress

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data